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外源油菜素内酯对弱光下番茄幼苗光合碳同化关键酶及其基因的影响

ISSN号：0513-353X
期刊名称：《园艺学报》
时间：0
分类：S641.2[农业科学—蔬菜学;农业科学—园艺学]
作者机构：南京农业大学园艺学院,南京210095
相关基金：国家现代农业产业技术体系建设专项资金项目（CARS-25-C-03）;江苏省农业三新工程项目（SXGC[2014]256,SXGC[2015]321,SXGC[2016]177）

关键词：番茄, 弱光胁迫, 油菜素内酯, 生长, 光合酶, 基因表达, tomoto, low light stress, brassinolides, growth, photosynthetic enzyme, gene expression

中文摘要：

以弱光敏感型番茄品种‘基尔斯’为试验材料,研究叶面喷施24–表油菜素内酯（EBR）对弱光胁迫下番茄幼苗生长、光合碳同化关键酶活性及基因表达的影响。结果表明,弱光胁迫下幼苗质量、茎粗和壮苗指数分别下降了70.5%、13.0%和77.7%,而株高增加了45.3%,说明外源EBR显著缓解了弱光胁迫对幼苗生长的抑制。弱光胁迫使番茄叶片净光合速率（Pn）降低42.8%,而喷施EBR后Pn提高到对照的72.0%。弱光胁迫导致番茄光合碳同化相关的核酮糖–1,5–二磷酸羧化/加氧酶（Rubisco）、果糖–1,6–二磷酸醛缩酶（Ald）、果糖–1,6–二磷酸酯酶（FBPase）和Rubisco活化酶（RCA）活性分别降低41.6%、31.3%、31.4%和19.6%,EBR处理后显著提高了这些酶的活性。同时弱光胁迫显著降低碳同化关键酶基因Rbc L、Rbc S、RCA、Ald、FBPase、SBPase、GAPDH、TK和PRK的表达量,而EBR显著提高这些基因的表达水平,其中SBPase和GAPDH的m RNA转录接近对照水平,TK和PRK的m RNA转录水平比对照提高了29%和37%。这些结果表明外源EBR通过调控弱光胁迫下番茄幼苗碳同化相关酶活性及其基因表达,提高叶片净光合速率,缓解弱光胁迫对植株的伤害,从而增强番茄幼苗的弱光耐性。

英文摘要：

In the present work,we investigated the effects of 24-epibrasinolide on growth,activities of photosynthetic enzymes and their gene expressions in low-light sensitive tomato‘Jiersi’leaves under low light stress. The results showed that low light stress significantly reduce the weight,stem diameter and seedling index by 70.5%,13.0% and 77.7% as compared to the control,respectively,but increased plant height by 45.3%. Application of EBR significantly alleviated the low light stress induced-inhibition of growth in seedlings. Under low light,the net photosynthetic rate（Pn）was reduced by 42.8% as compared with the control,while Pn increased by spraying EBR to 72.0% of the control. Low light stress significantly decreased of ribulose-1,5-bisphosphate carboxylase（Rubisco）,Rubisco activating enzyme（RCA）,fructose-1,6- bisphosphate aldolase（Ald）,and fructose-1,6-phosphatase（FBPase）by 41.6%,31.3%,31.4%,and 19.6%,respectively. EBR significantly increased these enzymes activities of photosynthesis under low light stress. Moreover,the m RNA expressions of Rbc L,Rbc S,RCA,Ald,FBPase,GAPDH,TK,SBPase,and PRK were significantly decreased. EBR significantly improve the low light stress-mediated changes of aforementioned m RNA transcripts,which m RNA transcription of SBPase and GAPDH towarded the control values. Transcriptional levels of TK and PRK under low light stress plus EBR were higher than the control ones. These results suggest that EBR could improve photosynthetic capacity through regulating gene expression and activity of key enzymes for carbon assimilation,thus confers tolerance to low light stress on tomato plants.