中文摘要：

为了分析不同花生四烯酸产量深黄被孢霉的差异表达基因与花生四烯酸含量的相关性，利用前期构建的不同花生四烯酸产量的深黄被孢霉消减cDNA文库，并采用反向斑点杂交技术，对该文库进行差异表达基因的鉴定，同时对鉴定的差异表达基因进行分析。结果获得了诱变前后深黄被孢霉的差异表达基因共55个。对55个阳性克隆进行测序和序列分析，结果显示25个基因在深黄被孢霉诱变前后差异表达，其中22个基因是霉菌或其他真菌基因的已知序列，其他3个基因无明显的相似序列。这些差异表达基因的鉴定可能与花生四烯酸的产生密切相关，这为进一步探讨深黄被孢霉生产花生四烯酸的分子机理研究提供一定的参考。

英文摘要：

In order to analyze the correlation of differences MortiereUa deep yellow arachidonic acid yield different gene expression and arachidonic acid content, different prebuilt arachidonic acid production MortiereUa subtracted cDNA library, reverse dot blot technique is used to differentiate the library to identify the gene expression, and gene expression analysis of the differences identified. The results obtained before and after mutagenesis Mortierella differentially expressed genes in common 55. Of the 55 positive clones are sequenced and sequence analysis. The results show that 25 genes differentially expressed before and after MortiereUa mutagenesis, of which 22 genes are known sequences of other fungi, mold or genes, the other three genes have no significant sequence similarity. The identification of differentially expressed genes may be associated with peanut produce arachidonic acid. The molecular mechanism of this peanut production to further explore the deep Mortierella arachidonic acid to provide a reference.