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中文摘要：
目的探讨Sysmex XN-9000全自动血细胞分析仪PLT-F通道在低值血小板测定中的临床意义。方法采用Sysmex XN-9000全自动血细胞分析仪的3种方法电阻抗法（PLT-I）、光学法（PLT-O）、荧光核酸染色法（PLT-F）进行血小板计数,从精密度、低值血小板及红细胞碎片干扰评价,并与使用抗CD61抗体的流式细胞术检测结果进行比较。结果与电阻抗法（PLT-I）、光学法（PLT-O）相比,荧光核酸染色法（PLT-F）的重复性最好,精确度较高;在红细胞碎片干扰实验中,PLT-F具有较强的抗干扰能力（高、低浓度组P〉0.05）,PLT-O次之（高浓度组P〈0.01,低浓度组P〉0.05）;低值血小板相关分析结果显示3种方法都得到良好的相关性,PLT-F法的准确性较高,尤其在低值血小板计数中。结论临床常规标本可以使用Sysmex XN-9000全自动血液分析仪的PLT-I法检测,当标本血小板数目异常或溶血时,建议使用PLT-O法或PLT-F法复查,必要时采用镜检法或流式细胞术。
英文摘要：
Objective To evaluate the accuracy of PLT-F channel on low platelet counting in the SysmexXN-9000 automated hematology analyzer. Methods Blood samples with low platelet concentration or those mixed with erythrocyte fragments were de-tected with SysmexXN-9000 automated hematology analyzer using three platelet count methods：PLT-F,PLT-I,and PLT-O. As comparison,the samples were labeled with CD61 antibody,and analyzed using flow cytometry. Results PLT-F method showed higher reproducibility and accuracy in comparison with PLT-I method and PLT-O method. Platelet count by PLT-F method were less affected by erythrocyte fragments than that by PLT-O method. There was a better correlation between the PLT-F system and the immunological reference method,despite of samples with low platelet concentration. Conclusions This study revealed that PLT-I method can accurately count platelets in the clinical routine blood samples. However ,blood samples with low platelet con-centration or those containing erythrocyte fragment should be reviewed by PLT-O or PLT-F methods ,even by microscopy or flow cytometry if necessary.
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