中文摘要：

目的建立酸枣仁Ziziphi spinosae Semen黄酮HPLC指纹图谱,并测定其中2种成分的含有量。方法以斯皮诺素为参照峰,建立10批样品的HPLC指纹图谱。酸枣仁黄酮甲醇提取物的分析采用Agilent TC-C_（18）色谱柱（4.6 mm×250 mm,5μm）;流动相乙腈-0.1%醋酸,梯度洗脱;体积流量1 m L/min;柱温30℃;检测波长280 nm。结果 HPLC指纹图谱中有10个共有峰,并鉴定出其中8个（维采宁-Ⅱ、牡荆素葡萄糖苷、异斯皮诺素、斯皮诺素、6＇＇＇-pyridyloylspinosin、6＇＇＇-对羟基苯甲酰斯皮诺素、6＇＇＇-阿魏酰斯皮诺素、6＇＇＇-对香豆酰斯皮诺素）。斯皮诺素和6＇＇＇-阿魏酰斯皮诺素分别在15.00-40.00μg和5.00-14.00μg范围内线性关系良好,平均加样回收率（RSD）分别为100.5%（1.6%）和100.4%（1.6%）。结论该方法准确、简单、可靠,可用于酸枣仁黄酮的质量控制。

英文摘要：

AIM To establish the HPLC fingerprints of flavonoids in Ziziphi spinosae Semen and to determine the contents of two constituents. METHODS With spinosin as a reference peak, the HPLC fingerprints of ten batches of samples were established. The analysis of methanol extract of flavonoids in Ziziphi spinosae ,Semen was performed on a 30 ℃ thermostatic Agilent TC-Clscolumn （4. 6 mm ×250 mm, 5 μm）, with the mobile phase comprising of acetonitrile-0. 1% acetic acid flowing at 1 mL/min in a gradient elution manner, and the detection wavelength was set at 280 nm. RESULTS There were ten common peaks in the HPLC fingerprints, eight of which （ vicenin- ]I, glucosylvitexin, isospinosin, spinosin, 6＂-pyridyloylspinosin, 6＂-p-hydroxybenzoylspinosin, 6＂-feruloylspinosin and 6＂-p-coumaroylspinosin） were identified. Spinosin and 6＂-feruloylspinosin showed good lin- ear relationships within the ranges of 15.00 -40. 00 μg and 5.00 - 14. 00 μg, whose average recoveries （RSDs） were 100. 5% （ 1.6% ） and 100. 4% （ 1.6% ）, respectively. CONCLUSION This accurate, simple and relia- ble method can be used for the quality control of flavonoids in Ziziphi spinosae Semen.