中文摘要：

目的：对BD FACSAriaIII流式细胞仪在应用中出现的故障原因进行分析、研究，为在科研工作中正确使用该仪器提供依据。方法：通过对2012-2013年实验血液学国家重点实验室技术平台BD FACSAriaIII流式细胞仪的使用情况进行统计，对使用过程中出现的常见故障和需要由厂商工程师现场解决的故障进行统计，总结出高发故障的原因及解决方法。结果：通过对BD FACSAriaIII流式细胞仪的故障进行分析，为高通量流式细胞分选仪的正确使用提供指导，提高仪器运行的稳定性。结论：正确的使用和良好的维护有助于保证BD FACSAriaIII流式细胞仪的正常运行，从而确保科研工作的顺利进行。

英文摘要：

Objective：To provide a basis for proper use of BD FACSAriaIII flow cytometer by analyzing the related causes of its malfunction.Methods： Through study of using BD FACSAriaIII flow cytometer in core facilities of State Key Laboratory of Experimental Hematology in 2012-2013, frequently occurred malfunction, solved malfunction by manufacturer’s engineers and their causes and solutions are summarized.Results： To provide guidance of using high throughput cell sorters and improve the stability of running the instrument.Conclusion： Proper use and good repair helps to guarantee the normal operation of BD FACSAriaIII flow cytometer.