中文摘要：

从一批水稻品种“中花11”组织培养苗里分离到一个矮化突变株“C6PS”，它的T2代群体株高呈现3：1分离。利用该群体矮化单株与“珍汕97”、“牡丹江8”构建2个F2群体F2（cz）、F2（cM），两个群体中高株与矮株均呈现3：1分离，证明该性状变异为单基因控制。“C6PS”表现型与已经报道的Dwarf1隐性突变体“d1”相似，以D，附近标记RM430检测F2（CZ）群体基因型，结果显示群体表型与RM430基因型呈极显著相关（P=0．0001），将该基因初步定位于Dwmf1附近。对“C6PS”及“中花11”进行D1序列分析显示，突变株中D1基因在其第九个外显予与第九个内含子的剪接位点上发生6个碱基的缺失，根据缺失两侧序列设计C6PS-D1L／R标记，在T2代群体该标记与表型呈现共分离，表明“C6PS”是一种新的Dwarf1突变体。cDNA测序显示突变体洲基因转录产物发生26个碱基的缺失，导致移码产生终止突变，从而无法翻译出有功能的Ga蛋白，因此，它是一个Ga功能缺失突变体。叶倾斜度检测显示“C6PS”对油菜素内酯响应比野生型“中花11”弱。

英文摘要：

A dwarf mutant C6PS, which has the similar phenotype as the recessive mutant Dwarfl （dl）, was produced from tissue-cultured plants of Zhonghua 11. In its progeny （T2）, the ratio of tall to dwarf plants was in agreement with the expected segregation ratio （3：1） of a single Mendelian inheritance gene, which indicated that the variation of plant height is caused by a single gene. To locate the mutation, C6PS was crossed with Zhenshan 97 and Mudanjiang 8 for producing two F2 populations of F2 （CM） and F2 （CZ）, respectively. The plant height in each F2 population also showed the same segrega- tion pattern as that in T2 generation. SSR marker RM430 closely linked to Dwarfl was preferentially used to genotype the F~ （CZ） population because C6PS showed the similar phenotype to dl mutant~ RM430 was significantly associated with plant height, which indicated that the mutant gene might be D1. Comparative sequencing of D1 between C6PS and Zhonghua 11 showed a 6 bp deletion occurred in the splice site of its ninth exon. The marker C6PS-D1L/R designed on the 6 bp deletion was co-segregated with plant height in T2 generation. The results indicated that C6PS was a new mutant of D 1 This mutation led to a 26 bp deletion of the transcript and resulted in a frame-shift mutation and a premature stop codon inC6PS, which could not translate the functional Get protein. C6PS was weakly sensitive to Brassinolide based on the leaf inclination angle test.