以单因素试验考察了微波功率、提取时间、提取温度、提取次数及料液比等因素对肠浒苔多糖提取量的影响,采用Design-Expert 8.0.5软件对微波辅助提取肠浒苔多糖的提取条件进行响应面法优化。结果表明,影响肠浒苔多糖微波辅助提取主要因素的主次顺序为:提取温度〉微波功率〉提取次数〉提取时间。肠浒苔多糖微波辅助提取的最佳工艺条件为:微波功率500 W,提取时间15 min,提取温度90℃,提取2次,料液比130(g/m L),粗多糖的得率为11.38%,该条件下测得的多糖含量为31.34%。可为肠浒苔多糖提取工艺的研究提供参考。
In order to optimize the conditions of microwave assisted extraction of polysaccharides from Enteromorpha intestinals,the effect of 5 factors including microwave power,including the extraction duration,temperature,times of extraction and solid / liquid ratio on the extraction yield,which was investigated by the single factors in experiment. The extraction conditions were further optimized by the response surface methodology with the Design-Expert 8. 0. 5 software. The results showed that their effects were in the order of temperature 〉microwave power 〉times of extraction 〉extraction duration. The optimal extraction conditions of microwave assisted extraction of polysaccharides from E. intestinals were determined as followed: microwave power 500 W,extraction duration 15 min,temperature 90 ℃,extracting 2 times,solid / liquid ratio of 130( g / m L). Under these optimal conditions,the experimental yield was 11. 38% with polysaccharides content of 31. 34%. It provides a reference in the research of extraction technology of E. intestinals polysaccharides.