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人工放流4龄中华鲟盐度适应过程中血清生化指标的变化

ISSN号：2095-1388
期刊名称：《大连海洋大学学报》
时间：0
分类：Q955[生物学—动物学]
作者机构：[1]华东师范大学生命科学学院,上海200062, [2]上海市长江口中华鲟自然保护区管理处,上海200092
相关基金：上海市长江口中华鲟保护区专项（沪鲟保研20090727001）; “十二五”国家科技支撑计划项目（2012BAD25B03）; 公益性行业（农业）科研专项（201203065）

作者：杨吉平[1,2], 陈立侨[1], 刘健, 李二超[1], 郑跃平, 崔超[1], 陈锦辉

关键词：中华鲟, 盐度胁迫, 血清, 生化指标, Acipenser sinensis, salinity stress, serum, biochemical parameter

中文摘要：

为研究人工放流4龄中华鲟Acipenser sinensis对长江口盐度的可能适应性,以淡水为对照,测定了在盐度为5、10急性胁迫下,鱼体在6、12、24、48、96 h时各种血液生化指标的含量。结果表明：与对照组相比,4龄中华鲟进入盐度为5的水中后,6 h时鱼血清中谷丙转氨酶（ALT）含量显著升高（P〈0.05）;12 h时,血清中血糖（GLU）、高密度脂蛋白胆固醇（HDL-C）含量显著升高（P〈0.05）,而ALT含量恢复至对照组水平;24 h时,尿素（UREA）含量显著降低（P〈0.05）,而GLU、HDL-C含量则恢复至对照组水平。4龄中华鲟进入盐度为10的水中后,6 h时鱼血清中总蛋白（TP）、ALT含量显著升高（P〈0.05）;12 h时,血清中TP、GLU、甘油三酯（TG）、HDL-C含量显著升高（P〈0.05）,而ALT含量则恢复至对照组水平;24 h时,血清中胆固醇（CHOL）、HDL-C含量显著降低（P〈0.05）,而TP、GLU、TG含量则恢复至对照组水平;48、96 h时,血清中HDL-C含量显著降低（P〈0.05）,而CHOL含量恢复至对照组水平。本研究表明,在4龄中华鲟盐度适应过程中,蛋白质可能优先于其他物质被动员而发挥调节作用,糖和脂肪则是机体重要的能量来源。

英文摘要：

In order to understand salinity adaptation of Chinese sturgeon Acipenser sinensis,some biochemical parameters were measured in serum of 4-years-old Chinese sturgeon exposed to freshwater（as the control） and a salinity of 5 and 10 brackish water at 6,12,24,48 h,and 96 h.The results showed that the serum alanine aminotransferase（ALT） activity was increased significantly in the fish exposed to a salinity of 5 at 6 h,compared with the control（P〈0.05）,and recovered to the control level at 12 h.The glucose（GLU）,and high-density lipoprotein cholesterol（HDL-C） contents were found to be significantly increased at 12 h（P〈0.05） and recovered to the control level at 24 h.However,urea（UREA） content was significantly decreased at 24 h（P〈0.05）.In the fish exposed to a salinity of 10,serum total protein（TP） content and ALT activity at 6 h,TP,GLU,triglyceride（TG）,and HDL-C contents at 12 h were significantly increased compared with the control（P〈0.05）.Total cholesterol（CHOL） content at 24 h,and HDL-C content at 24,48 h and 96 h were shown to be significantly decreased（P〈0.05）.ALT activity at 12 h,TP,GLU,and TG contents at 24 h,and CHOL content at 48 h were found to be recovered to the control levels.The findings indicate that serum protein plays a regulatory role with priority of carbohydrate and lipids which function importantly in energy supply during the adaptation to brackish water in 4-years-old Chinese sturgeon.

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