中文摘要：

本文介绍了3种常用的亚细胞结构成像技术包括荧光超分辨显微成像术、软X-射线显微镜和三维冷冻电镜的成像原理，细胞成像应用及所存在的问题。并提出多种显微镜联用的关联显微术由于结合了多种显微镜的优势，能够提供多方位信息，丰富细胞生物学的研究，有可能给细胞学带来全新的认识。

英文摘要：

This review focused on the developments of three common microscopes in cell biology including far-field light nanoscopy, soft X-ray microscopy based on the synchrotron radiation light source, and three-dimensional cryo-electron microscopy. Their mechanisms, applications in cell imaging and the existing problems are discussed. The correlative microscopy combining several imaging methods can supply more information and may provide a new insight into cell biology in the future.