中文摘要：

【目的】旨在阐明患脂肪肝奶牛体内代谢、内分泌以及肝和脂肪中3个基因表达的变化。【方法】分别对10头患脂肪肝奶牛和10头健康奶牛进行血液指标和肝脂含量的检测,利用半定量RT-PCR检测奶牛肝PEPCK-C mRNA的丰度,应用实时荧光定量PCR检测脂肪Lp mRNA和HSL mRNA的丰度。【结果】①脂肪肝奶牛血糖浓度显著降低（P＜0.01）,血浆NEFA和BHBA的浓度显著升高（P＜0.01）,肝脂（约41.98%左右）和血清AST活性显著增加（P＜0.01）,血清γ-GT、TBI、CHE显著升高（P＜0.05）;②脂肪肝奶牛Ins浓度、Ins/Gn明显升高（P＜0.05）,肝PEPCK-C和脂肪HSL mRNA表达水平显著降低（P＜0.05）;③脂肪Lp mRNA表达水平和血浆Lp、NPY浓度显著下降（P＜0.05）。【结论】患脂肪肝奶牛存在严重的能量代谢障碍和明显的肝功障碍,体内Ins与Gn、Lp与NPY之间失调,肝PEPCK-C和脂肪HSL、Lp的基因表达减弱,是病牛能量代谢障碍发生的重要机制。

英文摘要：

[Objective] To understand the changes of metabolism, endocrine and mRNA expression of three genes in liver and adipose of fatty liver cows. [Method] Ten fatty liver cows and ten health control cows were chosen from the same dairy cow farm were used in the experiment. Fourteen blood parameters and liver fat content were detected in the tested cows. Meanwhile, mRNA expression of liver PEPCK-C was measured by a semi-quantitative RT-PCR, of adipose Lp and HSL by a real time RT-PCR in the tested cows. [Result] In fatty liver cows, the concentration of plasma glucose decreased very significantly （P〈0.01）, plasma NEFA and BHBA increased very markedly （P〈0.01）, the liver fat content （about 41.88%） and activity of serum AST increased very significantly （P〈 0.01）, the level of serum TBI, γ-GT and CHE increased significantly （P〈0.05）, compared with the health control cows. In fatty liver cows, the concentration of plasma Ins and ratio of Ins to Gn all increased significantly （P〈0.05）, mRNA expression level of liver PEPCK-C increased markedly （P〈0.05）, and of adipose HSL also decreased significantly （P〈0.05）, compared with the health control cows. mRNA expression level of adipose Lp, the concentration of plasma Lp and NPY all were lower markedly in fatty liver cows than that in health control cows（P〈0.05）. [Conclusion] These results suggested that fatty liver cows have serious energy metabolism disorder, and marked liver disfunction. It might be an important mechanism of energy metabolic disorder for fatty liver cows to have a disturbance between Ins and Gn, Lp and NPY, and a decreased mRNA level of liver PEPCK-C, fat Lp and HSL.