中文摘要：

【目的】探讨淫羊藿甙诱导骨髓间充质干细胞（MSCS）向成骨细胞分化过程中对Wnt信号通路的影响。【方法】采用密度梯度离心法获得人骨髓间充质干细胞,采用含40滋g/L淫羊藿甙诱导液诱导其向成骨细胞分化,通过Western blot方法检测Wnt通道相关蛋白表达水平,荧光定量PCR方法检测Wnt通道相关基因表达水平。【结果】原代MSCs呈梭形,淫羊藿甙诱导12 d后淫羊藿甙组见明显结节形成,经细胞碱性磷酸酶染色,可见细胞内蓝色颗粒,细胞聚集区成紫黑色;21 d后茜素红染色可见红色结节堆积。MSCs经淫羊藿甙诱导21 d后,Wnt3a、茁-catenin蛋白表达较对照组显著增高,Wnt3a、茁-catenin mRNA表达较对照组显著上调（均P〈0.05）。【结论】淫羊藿甙可通过促进Wnt3a/茁-catenin信号通路促进MSCs向成骨细胞分化。

英文摘要：

Objective To investigate the effect of icariin on Wnt signal pathway during osteogenic differentiation of human bone marrow-derived mesenchymal stem cells （MSCs） . Methods The human bone marrow-derived MSCs were harvested by density gradient centrifugation method, and then were cultured in the medium containing 40 μg/L icariin to induce osteogenic differentiation. The expression levels of Wnt signaling pathway associated proteins and genes were detected by Western blotting method and fluorescence quantitative polymerase chain reaction, respectively. Results Primary MSCs were fusiforn~ After cocultured with icariin for 12 days, the features of osteogenic differentiation of MSCs were found in icariin group, and presented as blue cellular granules and crowded cells being purplish black after alkaline phosphatase staining. After cocultured with icariin for 21 days, red cell clusters were shown after alizarin red staining, and the expression levels of Wnt3a and β- catenin protein and mRNA were increased as compared with the control group（P〈0.05） . Conclusion MSCs with high purity can be harvested by density gradient centrifugation method, and icariin can promote osteogenic differentiation of MSCs through Wnt3a / β-catenin signal pathway.