中文摘要：

为快速建立优化的辣椒SRAP反应体系，利用L9（3^4）正交表，探讨了Mg2＋、dNTPs、引物和模板DNA4种主要反应成分的浓度变化对SRAP扩增结果的影响。结果表明：通过正交设计实验可高效建立优化稳定的辣椒SRXP反应体系；用该法建立的辣椒SRAP优化反应体系为：20μL反应体系中含10×PCRBuffer2．0μL、Mg2＋2．5mmol／L、dNTPs0．2mmol／L，上下游引物各0．4μmol／L、模板DNA10ng，TaqDNA聚合酶1U。

英文摘要：

To construct the optimum SRAP reaction system of pepper, orthogonal experimental design L9 （3^4 ） was used. And the comprehensive effect of three levels of four main factors（Mg2＋ , dNTPs, primers and DNA template） was assessed. The results showed that by orthogonal design, an optimal and stable SRAP system for pepper was quickly obtained：2. 5 mmol/L Mg2＋ , 0. 2 mmol/L dNTPs, 0. 4 μmol/L primers, 10 ng DNA template and 1 U Taq polymerase within total 20μL reaction solution.