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豨莶草提取物对抗多柔比星致大鼠急性肝肾损伤的作用及其机制

ISSN号：1004-0781
期刊名称：《医药导报》
时间：0
分类：R282.71[医药卫生—中药学;医药卫生—中医学] R285.5[医药卫生—中药学;医药卫生—中医学]
作者机构：[1] 河北医科大学第一医院药剂科,石家庄050031, [2] 河北医科大学基础医学院药理教研室,石家庄050017, [3] 河北省计划生育科学技术研究院生殖医学中心,石家庄050050
相关基金：河北省中医药管理局基金资助项目（2012008）;河北省自然科学基金资助课题（C2011206145）

作者：于静[1], 王建欣[1], 苏素文[2], 谢克让, 张玉, 杨继章[1], 苏悦[2]

关键词：豨莶草, 多柔比星, 损伤, 肝, 损伤, 肾, 氧化应激, Herba siegesbeckiae, Doxorubicin, Hepatic injury, Renal injury, Oxidative stress

中文摘要：

目的探讨稀莶草（HS）提取物对多柔比星（DOX）所致大鼠急性肝肾损伤的影响及其机制.方法将40只大鼠随机分为4组,各10只：HS提取物低、高剂量组分别灌胃给予HS提取物170和340 mg·kg-1,对照组、模型组灌胃给予等容积纯化水,连续7d.给药第5天,除对照组腹腔注射0.9％氯化钠溶液外,其他3组均腹腔注射DOX20 mg·kg-1.于第7天将大鼠麻醉后测定血清各种生化指标、过氧化产物及自由基的含量以及肝脏、肾脏组织过氧化产物及自由基的含量.结果与模型组比较,HS提取物高、低剂量组血清中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）及尿素氮（BUN）水平均较低,肝脏及肾脏组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）及丙二醛（MDA）水平均较模型组有所恢复.肝脏组织病理结果显示HS提取物高、低剂量组可不同程度缓解DOX所致的充血肿胀,肾脏组织病理结果显示HS提取物高、低剂量组可不同程度缓解DOX所致的炎细胞浸润及出血,都以高剂量组作用最为明显.结论 HS可明显减轻DOX所致大鼠急性肝肾损伤,其作用机制可能是由于HS提取物能增强机体的抗氧化应激能力.

英文摘要：

Objective To investigate the potential protective effect of extracts from Herba siegesbeckiae （ HS） onhepatic and renal injury induced by doxorubicin（DOX） in rats. Methods Male Sprague-Dawley rats were randomly dividedinto 4 groups with 10 in each. Rats in the low and high dose groups were intragastrically administered with HS extract at the dosesof 170 and 340 mg·kg-1 ·d-1 , respectively, for 7 consecutive days. Rats in the control and model groups were given with equalamount of water. On the 5th day, all groups were given with DOX （20 mg·kg-1 ） intraperitoneally except the control, which wasgiven equal amount of saline. On the 7th day, evaluation of hepatic and renal protection effects by HS extract was performed byanalyzing the serum biochemical indicators, contents of preoxidation（MDA）, and free radicals in both serum and tissues of theliver and the kidneys. Results Pretreatment with HS showed significant decrement of serum BUN、ALT and AST levels in adose-dependent manner compared with DOX group. The increment of MDA production and decrements of SOD and CAT caused byDOX were also recovered dose dependently in HS treated groups. The hepatic cells in the DOX group showed obvious congestionand swelling, whereas the symptoms in HS group were significantly relieved in a dose dependent manner. The renal interstitial inthe DOX group showed obvious congestion and swelling, however the symptoms in HS group were significantly relieved. There wasno obvious congestive swelling phenomenon in high dose of HS group. Conclusion HS has protective effect against DOX-induced hepatic and renal toxicities in rats which may be caused by its anti-oxidative effect.

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