中文摘要：

目的复制肾虚胚胎着床障碍小鼠病证结合模型并对其进行评价。方法应用羟基脲、米非司酮造模,选择L9（34）表安排实验,根据羟基脲及米非司酮不同剂量配比组合及对照组共10组,检测各组模型的超氧化物歧化酶（SOD）、雌二醇（E2）、孕激素（PRG）及体质量等指标,并观察小鼠精神状态、行为、大小便、毛发色泽、反应情况等肾虚症状。结果研究范围内,当羟基脲取一水平、米非司酮取一水平时,妊娠第5天时血清中SOD、E2、PRG均是最少的,妊娠第8天时血清中E2及PRG是最少的。随着药物的代谢和怀孕动物自身机制调节,羟基脲和米非司酮各自对怀孕动物血清中雌二醇水平、孕激素的抑制力大为减弱。在肾虚胚胎着床障碍小鼠模型的复制中,羟基脲的贡献度最大,米非司酮的贡献度次之,一定程度上,SOD、E2、PRG与羟基脲和米非司酮的用量成反比。结论综合多项指标,在研究范围内,AⅠBⅠ较为合适作为肾虚胚胎着床障碍小鼠病证结合模型,既能反应胚胎着床障碍西医病的特点,又能反映中医肾虚证候特征。

英文摘要：

【Objective】 To ascertain the optimal combination of Hydroxycarbamide and Miferristone in the mouse model of embryo implantation dysfunction（EID） with kidney deficiency syndrome. 【Methods】 Taken Hydroxycarbamide, Miferristone as influencing factors, according to the L9（34） orthogonal array, mathematical model was erected to observe the effects of different combination of Hydroxycarbamide and miferristone in the mouse model of EID with integrated disease and syndrome. 【Results】 Within the study, when Hydroxyurea to second level and Miferristone to one level, in the first five days of pregnancy, SOD, E2, PRG in the blood serum and uterus were least, the number of independent activities were all least. In the first eight days of pregnancy, E2, PRG in the blood serum were least. Hydroxycarbamide took the greatest contribution, and Miferristone fellow the contribution to the mouse model of embryo implantation dysfunction（EID） with kidney deficiency syndrome. To a certain extent, SOD, E2, PRG in the blood serum and uterus were in inverse proportion to the dose of Hydroxycarbamide and Miferristone. 【Conclusion】 Through erecting the ratio of its mathematical model by the orthogonal designs, the optimal proportion of Hydroxycarbamide and Miferristone in the mouse model of EID with integrated disease and syndrome, an objective forecast can be made. Integrated a number of indicators, within the scope of the study, AⅠBⅠ was a more appropriate model of EID with kidney deficiency syndrome for further drug intervention study.