中文摘要：

目的：利用Notehl的胞内段（Notchl intracellular domain，NICD）基因过表达载体建立稳定过表达NICD的人牙髓干细胞（dental pulp stem cells，DPSCs）株，观察NICD过表达对DPSCs增殖、迁移能力的影响。方法：构建含NICD过表达的慢病毒颗粒，并将其转染至DPSCs构建过表达NICD的细胞株（DPSCs／NICD）；以DPSCs／NICD为实验组，正常细胞株（DPSCs／wt）和空病毒转染的空载组（DPSCs／vector）作为对照，通过RT—PCR和Western Blot检测NICDmRNA及其蛋白的表达；细胞免疫荧光观察NICD在DPSCs上的表达位置；CCK8法检测细胞的增殖情况；流式细胞技术检测细胞周期中S期的改变；Transwll检测细胞的迁移能力。结果：与DPSCs／wt组和DPSCs／vector组相比，DPSCs／NICD组的NICDmRNA及其蛋白表达水平均明显增强（P〈0．05），表达位置在胞膜、胞浆及胞核；细胞的增殖速度加快、细胞周期中S期比例升高、迁移能力增强。结论：NICD过表达使DPSCs细胞的增殖和迁移能力增强。

英文摘要：

AIM： To observe the effects of Notch l intracellular domain（NICD） overexpression on the proliferation and migration of human dental pulp stem cells （DPSCs）. METHODS ： NICD over-expressing lentivirus was constructed. DPSCs were transfected with the NICD over-expressing lentivirus. DPSCs/NICD cells, non-transfected cells （DPSCs/wt） and vector transfected ceils （DPSCs/vector） were cultured respectively, mRNA and protein expression of NICD in the cells was determined by RT-PCR and Western blot respectively. Cell proliferation was observed with CCK8 colorimeter. Cell cycle distribution was detected by flow cytometry. Cell migration was detected by Transwll method. RESULTS ： NICD mRNA and protein expression was higher in DPSCs/NICD cells than in DPSCs/wt and DPSCs/vector cells （P 〈 0.05）. DPSCs/NICD cells showed faster proliferation, stronger migration ability and more cells in S phase. CONCLUSION： NICD overpression may enhance cell proliferation and migration of DPSCs.