中文摘要：

目的利用基质辅助激光解析电离飞行时间质谱（MALDI—TOF—MS）构建标准沙门菌株数据库。方法利用MALDI—TOF—MS对21株标准沙门菌进行质谱数据采集，建立标准菌株数据库。相关菌株质谱数据通过菌株在相同培养环境不同培养时间下所得质谱数据进行优化。通过血清型鉴定方法筛选出200株野生沙门茵，并利用自建数据库和现有数据库对200株野生沙门菌进行MALDI-TOF—MS检测，对自建数据库进行评价。结果Bruker原有数据库、自建数据库及合并数据库对200株野生沙门菌鉴定，阳性率分别为87．5％、97．0％、97．5％。21株标准沙门菌株数据库按亲缘关系远近可分为3大类。主成分分析发现不同菌种之间质谱数据有一定的差异，个别菌种在不同培养条件下培养，质谱数据变化不明显。结论本研究建立的标准菌库对野生沙门菌的鉴定准确性高，适用于实际检测。研究结果丰富了已有的数据库，同时也为其他细菌菌库的建立提供了很好的借鉴。

英文摘要：

Objective To establish the mass spectrometry database of Salmonella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Methods By use of standard stains representing 21 species of Salmonella, a reference database was established MALDI-TOF-MS-based species identification. The profiles of colonies in the identical culture condition with different times were analysed for each of standard strains. To evaluate the database, 200 blind-coded isolates were tested by the established database and the original database. Results The positive rate of identification by the original database, the established database and conbine-database were 87.5%, 97.0% and 97.5%. 21 Salmonella strains were divided into 3 types by MALDI-TOF-MS at distance level of 500. PCA of the reference strains showed difference between them. There were little changes of mass spectrometric data of several strains in different culture condition. Conclusion The established database could expand the original one and be used for identification of Salmonella species. The strategy may be fit for other bacteria.