中文摘要：

目的观察白藜芦醇对TNF-α刺激引起的内皮细胞炎症反应的影响,并围绕TNFR1探索相关作用机制。方法不同浓度（0.01、0.1、1、2.5、5、10、20、50、100、200、400 ng/ml）TNF-α处理人血管内皮细胞株EA.hy926,24 h后MTT法和ELISA法分别检测细胞增殖活力和细胞培养液中sICAM-1释放水平;白藜芦醇（浓度为0.1、1、10μmol/L）预处理24 h后,再用TNF-α（10 ng/ml）处理24 h,利用ELISA法检测细胞培养液中sICAM-1释放水平,qRT-PCR法检测ICAM-1mRNA和TNFR1 mRNA表达水平。结果高浓度TNF-α（≥100 ng/ml）刺激EA.hy926细胞后,细胞增殖活力显著下降（P〈0.05）,当TNF-浓度为200 ng/ml时,与对照组比较细胞增殖活力下降约59.7%;浓度为10 ng/ml的TNF-α刺激内皮细胞后,细胞的ICAM-1及TNFR1 mRNA表达明显上调（P〈0.05）;白藜芦醇预处理内皮细胞后可明显下调TNFR1 mRNA表达水平（P〈0.05）,并显著抑制TNF-α诱导的ICAM-1的表达（P〈0.05）,且抑制作用随着浓度的增加而增强。结论白藜芦醇可能通过抑制TNFR1表达,进而抑制TNF-α刺激引起的炎性因子释放,减轻内皮炎性损伤。

英文摘要：

Objective To determine the inhibitory effects of resveratrol on TNF-α induced inflammatory damage in vascular endothelial cell line EA. hy926. Methods After the endothelial EA. hy926 cells were treated with TNF-α of different concentrations （0.01, 0.1,1, 2.5, 5, 10, 20, 50, 100, 200, and 400 ng/ml） for 24 h, cell viability and slCAM-1 release in the supernatant were measured by MTT and ELISA assay, respectively. Cells were pretreated with resveratrol of different concentrations （0.1, 1, and 10 p, mol/L） for 24 h, and then incubated with TNF-a （ 10 ng/ml） for another 24 h. sICAM-1 level in the the supernatant was determined by slCAM-1 ELISA kit. The mRNA expression of intercellular adhesion molecule-1 （ICAM-1） and tumor necrosis factor receptor-1 （TNFR1） were measured by qRT-PCR assay. Results After treated with TNF-α at high concentration （≥ 100 ng/ml）, cell viability was decreased significantly. Cell viability incubated with 200 ng/ml TNF-α was decreased by about 59.7% compared with control. When treated with a low concentration of TNF-α （ 10 ng/ml ） , the mRNA levels of ICAM-1 and TNFR1 were increased markedly （P 〈0.05 ）. However, resveratrol pretreatment significantly （P 〈 0. 01 ） inhibited the increase of ICAM-1 and TNFR1 mRNA expression induced by TNF-α in a dose-dependent manner. Conclusion Resveratrol might inhibit the TNF-α-induced inflammatory cytokine release and alleviate endothelial inflammation by inhibiting TNFRl-mediated signaling pathway. atherosclerosis ; resveratrol ; EA. hy926 ; inflammation ; intercellular adhesion molecule-1 ; tumor necrosis factor receptor-1