中文摘要：

流动 cytometry， BrdU 加入，并且西方的弄污被用来调查 caveolin-1 (Cav-1 ) 是否涉及肾的 glomerular mesangial 房间的房间周期前进。宽类型 mesangial 房间与 20% 胎儿的牛的浆液(FBS ) 在 22 h 孵化以后重入房间周期， Cav-1 猛烈房间在 48 h 以后留在 G0/G1 阶段，它显示 Cav-1 猛烈 mesangial 房间是 G0/G1 拘捕。cyclin D1 的蛋白质水平显著地在 Cav-1 猛烈房间在宽类型 mesangial 房间，和 12 h 为 6 h 与 20% FBS 在孵化以后增加了。它建议 cyclin D1 upregulation 在猛烈 mesangial 房间被推迟。在 Cav-1 猛烈 mesangial 房间的 Cav-1 重新表示从 4.8% ～ 15.26% 增加了 S 阶段的比率，并且减少 G0/G1 的比率从 90.96% ～ 77.84% 分阶段执行，它暗示 Cav-1 重新表示能逆行房间周期拘捕。它断定 Cav-1 可以支持 mesangial 房间的增长。

英文摘要：

Flow cytometry,BrdU incorporation,and western blotting were used to investigate whether caveolin-1（Cav-1） is involved in cell cycle progression of renal glomerular mesangial cells.Wide type mesangial cells re-entered the cell cycle after 22 h incubation with 20% fetal bovine serum（FBS） and Cav-1 knockout cells remained in G0/G1 phase after 48 h,which indicated that Cav-1 knockout mesangial cells were G0/G1 arrest.The protein level of cyclin D1 significantly increased after incubation with 20% FBS for 6 h in wide type mesangial cells,and 12 h in Cav-1 knockout cells.It suggested that cyclin D1 upregulation was delayed in knockout mesangial cells.Cav-1 re-expression in Cav-1 knockout mesangial cells increased the ratio of S phase from 4.8% to 15.26%,and decreased the ratio of G0/G1 phase from 90.96% to 77.84%,which implied that Cav-1 re-expression can reverse cell cycle arrest.It concludes that Cav-1 may promote the proliferation of mesangial cells.