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大气汞浓度升高对水稻叶片生理效应的影响研究
  • ISSN号:1673-5897
  • 期刊名称:《生态毒理学报》
  • 时间:0
  • 分类:X171.5[环境科学与工程—环境科学]
  • 作者机构:[1]中国科学院生态环境研究中心大气环境科学实验室,北京100085, [2]中国科学院大学资源与环境学院,北京100049, [3]湖南农业大学资源环境学院,长沙410128
  • 相关基金:国家自然科学基金项目(No.41373124,41073092); 国家重大基础研究(973)计划项目(2013CB430002)
中文摘要:

采用大田开顶式气室熏气实验,研究大气汞浓度升高对水稻叶片气体交换参数、脯氨酸、丙二醛的积累以及超氧化物歧化酶活性的影响。实验结果显示,水稻叶片净光合速率(Pn)和气孔导度(Gs)随大气汞浓度的升高均较对照略微下降,表明大气汞浓度的升高对水稻光合作用和气孔开放程度有一定影响;扬花期水稻胞间CO2浓度(Ci)随大气汞浓度的升高明显降低(P〈0.05)表明Pn的略微下降属于气孔限制,同时蒸腾速率(Tr)显著增加(P〈0.01)表明大气汞对水稻的蒸腾生理功能有一定的影响。乳熟期水稻叶片气体交换参数与大气汞浓度无显著差异(P〉0.05),且各指标均低于扬花期。水稻叶片脯氨酸(Pro)含量在拔节期随大气汞浓度的升高而显著增加(P〈0.05),扬花期先升高后下降,在45 ng·m^-3时达到最大,成熟期无显著差异(P〉0.05);水稻叶片丙二醛(MDA)含量在拔节期先升高后下降,45 ng·m^-3时达到最大,扬花期和成熟期均无显著差异(P〉0.05);水稻叶片超氧化物歧化酶(SOD)活性在拔节期先升高后下降,15 ng·m^-3时达到最大,扬花期无显著差异(P〉0.05)。以上结果表明大气汞浓度的升高可以引起水稻叶片膜脂过氧化以及脯氨酸和丙二醛含量的积累,且随着体内Pro、MDA和SOD对大气汞胁迫的协同反应,水稻对逆境的适应能力增强,对汞胁迫产生了耐受性。

英文摘要:

The effects of elevated gaseous elemental mercury( GEM) on gas exchange parameters,accumulation of proline( Pro) and malondialdehyde( MDA),activity of superoxide dismutase( SOD) in rice foliage were studied with field open-top chambers( OTCs) fumigation experiment. The results showed that the net photosynthesis rate( Pn) and stomatal conductance( Gs) were less slightly in GEM treatment than those in the control,which indicating that elevated GEM had some effect on photosynthesis and stomatal openness of rice leaves. In flowering stage of rice,the distinct decrease( P〈0. 05) of intercellular CO_2 concentration( Ci) with elevated GEM indicated that stomatal limitation led to the slight decrease of Pn,and the significant increase( P〈0. 01) of transpiration rate( Tr) with elevated GEM showed that the physiological function of rice transpiration was effected by Hg in the atmosphere. Gas exchange parameters of rice leaves in milky stage were insignificantly difference with GEM in air( P〉0. 05) and lower than that in flowering stage. Proline concentrations in rice foliage were increased obviously with elevated GEM( P 0. 05) in jointing stage,declined after increasing and reached to the maximum value at 45 ng·m-3 in flowering stage,and it was no significant difference( P〉0. 05) in mature stage among four treatments. The contents of MDA in rice foliage increased first and reached the highest value at 45 ng·m-3,and then decreased in jointing stage,and it was no significant difference( P 0. 05) with the increase of GEM in flowering and mature stage. The activity of SOD in rice foliage also increased first and then declined at 15 ng·m-3in jointing stage,and there was no significant difference( P〉0. 05) in flowering stage. These results suggested that elevated GEM in air can cause membrane lipid peroxidation and accumulation of Pro and MDA in rice foliage,furthermore the ability of adapting to adversity and the tolerance to elevated GEM for rice were

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期刊信息
  • 《生态毒理学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国科学院生态环境研究中心
  • 主编:王子健
  • 地址:北京市海淀区双清路18号北京2871信箱
  • 邮编:100085
  • 邮箱:stdlxb@rcees.ac.cn
  • 电话:010-62941072
  • 国际标准刊号:ISSN:1673-5897
  • 国内统一刊号:ISSN:11-5470/X
  • 邮发代号:2-303
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),美国生物科学数据库,英国动物学记录,中国中国科技核心期刊,中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:4571