中文摘要：

目的制备含多壁碳纳米管／聚乙烯亚胺（MWCNTs—PEI）复合物的壳聚糖/β-甘油磷酸钠（CS/β-GP）温敏凝胶，为双缓释载体的研究提供一定基础。方法以壳聚糖温敏凝胶为载体，将多壁碳纳米管／聚乙烯亚胺分散到温敏凝胶中，制备含多壁碳纳米管／聚乙烯亚胺复合物的壳聚糖温敏凝胶，以胶凝时间为指标，考察β-甘油磷酸钠浓度、pH、温度和多壁碳纳米管／聚乙烯亚胺复合物质量对壳聚糖温敏凝胶的影响，采用扫描电镜（SEM）、红外光谱（FTIR）等对其表征，并初步考察其体内相容性。结果流变法测定其胶凝温度大约为37．0℃，在一定范围内，壳聚糖温敏凝胶胶凝时间随β-甘油磷酸钠浓度、pH、温度以及多壁碳纳米管／聚乙烯亚胺复合物质量的增加而缩短，且在动物体内均可形成凝胶，通过扫描电镜和红外光谱等实验发现，加入多壁碳纳米管／聚乙烯亚胺复合物后，其不与壳聚糖温敏凝胶发生化学反应，且壳聚糖温敏凝胶孔洞明显变小，结构致密，最终导致溶胀率和溶蚀速率的减小。结论含多壁碳纳米管／聚乙烯亚胺复合物的壳聚糖／β-甘油磷酸钠温敏凝胶具有快速凝胶化和良好的温敏性，可以作为良好的双缓释性载体。

英文摘要：

OBJECTIVE To synthesis chitosmn/β-glycerophosphate （CS/β-GP） thermosensitive hydrogel containing multi-walled carbon nanotubes-polyethyleneimine （MWCNTs-PEI） complexes and to lay a foundation for further research of dual slow-release deliv- ery system. METHODS Chitosan thermosensitive hydrogel containing MWCNTs-PEI was prepared by MWCNTs-PEI dispersed to the chitosan thermosensitive hydrogel. As the indicator of the gelling time, the experiment studied the effect of β-GP concentration, pH, temperature and MWCNTs-PEI composite quality on the theermosensitive chitosan hydrogel, and then it was charactered by using trans- mission electron microscopy （SEM）, infrared spectrometer （IR）, and initially investigated in vivo compatibility. RESULTS The dy- namic rheology method investigated the gelling temperature were about 37.0 ℃. Within a certain range, the gelling time of thermosen- sitive chitosan hydrogel was shortened with the increase of concentration of β-GP, pH, temperature, and the quality of MWCNTs-PEI complexes, and they could be transtormed into the hydrogel in vivo. The addition of MWCNTs-PEI complex didn＇t react chemically with the thermosensitive chitosan hydrogel and significantly make the holes of the chitosan thermosensitive hydrogel smaller by SEM and FT- IR, eventually leading to the swelling rate and the corrosional ratio decrease. CONCLUSION Chitosan/β-glycerophosphate thermo- sensitive hydrogel containing amino-carbon nanotubes has a rapid gelation and good temperature-sensitivity, which can serve as a good double sustained-release carrier.