中文摘要：

目的：观察独活寄生汤含药血清对白细胞介素1β（interleukin-1 beta,IL-1β）诱导的退变关节软骨细胞中基质金属蛋白酶（matrix metalloproteinase,MMP）和环氧化酶2（cyclooxygenase 2,COX-2）表达的影响。方法：将36只2月龄SD大鼠随机分为独活寄生汤血清组、壮骨关节丸血清组和空白血清组,每组12只。分别以独活寄生汤、壮骨关节丸溶液和生理盐水灌胃,每次0.6 m L,每天2次,连续7 d。末次给药后采血,分离血清备用。将体外培养的SD大鼠第3代关节软骨细胞分为空白组、模型组、独活寄生汤组和壮骨关节丸组。模型组、独活寄生汤组和壮骨关节丸组软骨细胞加入IL-1β干预24 h后,分别采用含10%空白血清组血清、独活寄生汤血清组血清和壮骨关节丸血清组血清的DMEM培养基培养;空白组软骨细胞不加IL-1β,直接以含10%空白血清组血清的DMEM培养基培养。培养72 h后采用Western Blot法测定各组细胞中MMP-1、MMP-3、MMP-9、MMP-13和COX-2的含量。结果：4组软骨细胞的MMP-1、MMP-3、MMP-9、MMP-13和COX-2含量比较,组间差异均有统计学意义（0.135±0.007,0.324±0.006,0.174±0.007,0.234±0.007,F=266.333,P=0.000;0.150±0.028,0.346±0.027,0.250±0.028,0.293±0.028,F=26.855,P=0.000;0.131±0.014,0.283±0.009,0.148±0.014,0.158±0.015,F=50.442,P=0.000;0.173±0.035,0.691±0.051,0.318±0.038,0.286±0.039,F=91.860,P=0.000;0.201±0.033,0.796±0.031,0.370±0.033,0.327±0.034,F=125.270,P=0.000）。空白组的MMP-1、MMP-3、MMP-9、MMP-13和COX-2含量均低于模型组（P=0.000,P=0.000,P=0.000,P=0.000,P=0.000）。模型组的MMP-1、MMP-3、MMP-9、MMP-13和COX-2含量均高于独活寄生汤组和壮骨关节丸组（P=0.000,P=0.003,P=0.000,P=0.000,P=0.000;P=0.000,P=0.047,P=0.000,P=0.000,P=0.000）。独活寄生汤组的MMP-1含量低于壮骨关节丸组（P=0.000）;2组的MMP-3、MMP-9、MMP-13和COX-2含量比较,组间差异均无统计学意义（P=0.094,P=0.497,P=0.380,P=0.220）。结论：

英文摘要：

Objective：To observe the impact of Duhuo Jisheng Tang（独活寄生汤,DHJST） medicated serum on expression of matrix metalloproteinase （MMP）and cyclooxygenase 2 （COX- 2 ）in degenerative articular chondrocytes induced by interleukin -1 beta（ IL- 1β）. Methods：Thirty- six 2 -month- old SD rats were randomly divided into DHJST serum group, Zhuanggu Guanjie Wan（ 壮骨关节丸,ZGGJW ）serum group and blank serum group, 12 cases in each group. The rats were intragastric administrated with DHJST, ZGGJW solution and normal saline respectively（0.6 mL at a time, twice a day）for 7 consecutive day. After the last dose, the blood was collected and the ser- um was separated for further use. The third - generation articular chondrocytes of SD rats cultured in vitro were divided into blank group, model group, DHJST group and ZGGJW group. The chondrocytes in the latter 3 groups were cultured in Dulbecco＇ s Modified Eagle Medium （DMEM） supplemented with 10% serum of blank serum group, DHJST serum group and ZGGJW serum group after 24 -hour intervention with IL - 1β. The chondrocytes in blank group were cultured in DMEM supplemented with 10% serum of blank serum group and didn＇ t in- tervented by IL - 1β. The contents of MMP - 1, MMP - 3, MMP - 9, MMP - 13 and COX - 2 in the chondrocytes of each group were meas- ured by using Western Blot after 72 -hour culture. Results ：There was statistical difference in the contents of MMP- 1, MMP- 3, MMP- 9, MMP- 13 and COX- 2 in the chondrocytes between the 4 groups （0. 135 ＋/-0. 007,0. 324 ＋/-0. 006,0. 174 ＋/-0. 007,0. 234 ＋/- 0.007, F = 266. 333, P = 0. 000 ; 0. 150 ＋/- 0. 028,0. 346 ＋/- 0. 027,0. 250 ＋/- 0. 028,0. 293 ＋/- 0. 028, F = 26. 855, P = 0. 000 ; 0. 131 ＋/-0.014,0.283 ＋/-0.009,0. 148 ＋/-0. 014,0. 158 ＋/-0. 015,F =50. 442,P =0. 000;0. 173 ＋/-0. 035,0. 691 ＋/-0. 051, 0. 318 ＋/-0. 038,0.286 ＋/-0.039, F = 91. 860, P = 0. 000 ;0. 201 ＋/-0. 033,0. 796 ＋/-0. 031,0. 370 ＋/-0. 033,0. 327 ＋/-0. 034, F = 125.270