中文摘要：

建立一种快速、准确测定生物样品中左旋多巴（L-DOPA）、去甲肾上腺素（NE）、肾上腺素（E）、多巴柯（DOPAC）、多巴胺（DA）、5-羟吲哚乙酸（5-HIAA）、高香草酸（HVA）及5-羟色胺（5-HT）8种递质含量的高效液相色谱．电化学检测方法。使8种物质在25rain于单一流动相、单流速、单通道检测器情况下达到良好的分离效果。采用ESAMD-150色谱拄（150mm×3．2mm，3μm），流动相为50mmol／L柠檬酸、50mmol／L无水乙酸钠、0．5mmol／L1．庚烷磺酸钠、0．5mmol／L乙二胺四乙酸二钠、5mmol／L三乙胺，pH3．5，在甲醇浓度为5％～10％，流速0．3～0．5mL／min，柱温为30℃时，都能使8种物质很好分离，其中在甲醇浓度8％，流速0．4mL／min，检测到前5种物质线性范围为0．005～10nmol／L；后3种0．001～10nmol／L，8种物质相关系数往0．994～0．999之间，检出限在pmol／L水平；回收率在80．3％～102．1％之间，相对偏差在1．4％～4．8％之侧。且对样本处理和保存方法进行了探讨。

英文摘要：

A simple, rapid and sensitive HPLC-ECD method was established to detect the monoamine neuro- transmitters and its metabolites, eight neurotransmitters including L-Dihydroxyphenylalanine（L-DOPA） , Norepinephrine（ NE ） ; Eoubeogrube （ e ） ; 3,4-Hydroxyphenylacitie acid （ DOPAC ） ; 3-Hydroxytyramine hydro- chloride （ DA ） ; 5-Hydroxy-3-indoleacetic acid （ 5-HIAA ） ; 4-Hydroxy-3-methoxyacetie acid （ HVA ） ; 5-Hydroxytryamine（5-HT） can be separated and detected under the same mobile phase, flow rate and mono-channel detector in 25 rain. The analytes were separated on a ESA MD-150 column （150 mm× 3.2 ram, 3μm）. The mobile phase（ pH 3.5 ）, consisted of methanol-water phase （containing 50 mmol/L citric acid, 50 mmol/L anhydrous sodium acetate, 0. 5 mmol/L 1-octanesulfonie acid sodium salt, 0. 5 mmol/L Na2EDTA, 5 mmol/L Triethylamine, 5% -10% methanol）the column temperature was kept at 30 ℃. The eight transmitters can be separated at a flow rate of 0.3 -0.5 mL/min using 8% methanol with a flow rate of 0.4 mL/min. The first five components were observed be linear over a concentration range of 0. 005 - 10 nmol/L, and the followed three components 0. 001 - 10 nmol/L. The low limit of detection was the level of pmol/L. The correlation coefficients of these compounds were 0.994-0.999, the recoveries were 80.3% -102.1% , the RSDs were 1.42% -4.85%. The pretreatment of samples was also studied.