中文摘要：

目的：分别以胶原锚定方法修饰的聚乳酸-聚羟基乙酸共聚物（PLGA）为组织工程软骨支架材料，以脱细胞骨基质为组织工程骨支架材料，将二者结合制备出结合良好的组织工程骨软骨双层支架，并观察结构特征，以评估其作为组织工程化骨软骨复合体支架材料的可行性。方法：实验于2006—02／2007—02在解放军总医院骨科研究所完成。①支架的制备：以犬新鲜松质骨为原料，制备脱细胞骨基质作为骨支架材料；将脱细胞骨基质浸于盛有PLGA溶液的模具中，采用固-液相分离法结合致孔剂溶出法制备出多孔的PLGA／脱细胞骨基质双层支架，然后对PLGA支架部分进行等离子体处理和Ⅰ型胶原锚定修饰。得到的新型组织工程骨软骨双层支架的上层为多孔PLGA，下层为脱细胞骨基质。②支架的特征观察：对支架行扫描电镜检测，并采用乙醇置换法测定PLGA层孔隙率，采用北京亚林公司提供的扫描电镜图像分析系统测定PLGA层支架的平均孔径。结果：扫描电镜显示双层支架的PLGA部分具有良好的孔隙贯通结构，孔径为（211±33）μm，孔隙率为（95．0±1．5）％；脱细胞骨基质骨支架部分具有天然骨的孔径和空隙率；PLGA材料渗入骨支架部分，双层支架结合良好。结论：等离子体处理后胶原锚定修饰的PLGA／脱细胞骨基质双层支架具有良好的结构和孔隙率，结合良好，可作为支架载体应用于组织工程骨软骨复合体的构建。

英文摘要：

AIM： Taking collagen anchorage-modified poly（lactic-co-glycolic acid） （PLGA） as tissue engineered cartilage scaffold and acellular bone matrix （ACBM） as tissue engineered bone scaffold, this study was aimed to develop a bilayered scaffold using PLGA/ACBM, investigate its performance and assess its feasibility for tissue engineering osteochondral scaffold materials. METHODS： The experiment was carried out in the Orthopedics Institute of Chinese PLA at General Hospital of Chinese PLA between February 2006 and February 2007. （1）ACBM was prepared for tissue engineering bone scaffolds material using canine cancellous bone. After the ACBM scaffolds were placed into cylindrical moulds containing PLGA solution, a bilayered porous PLGA/ACBM scaffold was fabricated using the porogen leaching method combined with solid-liquid phase separation, then PLGA was modified by combining plasma pre-treatment and collagen anchorage. The upper layer of the novel bilayered scaffold was porous PLGA, and the lower layer was ACBM. （2）The scaffold was investigated by scanning electron microscope （SEM）. PLGA porosity was detected by using ethanol displacement method, and average pore diameter was determined by using SEM image analysis system, which was offered by Beijing Yalin Company. RESUTS： SEM results revealed that, PLGA scaffold had good pore interconnectivity with （211±33） μm pore diameter and （95.0±1.5）% porosity, and the ACBM scaffold had the natural pore and porosity. The two layers integrated very well. CONCLUSION： The porous PLGA/ACBM bilayered scaffold modified by plasma pre-treatment and collagen anchorage has good pore diameter and porosity, with the two layers integrate well, so it is potential for constructing tissue engineering osteochondral scaffold.