中文摘要：

α-乳清蛋白和β-乳球蛋白系由牛乳清提取的乳糖制品中的主要蛋白杂质，是引起食物及药物过敏的主要过敏原。因此，在食品安全及医药安全领域，对二者的分离及痕量检测是至关重要的。本研究建立了一种简单、快速、灵敏、重现性好的毛细管区带电泳（CZE）方法，使用简单的电泳体系和样品处理步骤，实现了α-乳清蛋白和β-乳球蛋白的基线分离和痕量检测。CZE条件如下：25mmol／L磷酸盐缓冲液（pH7．0），分析电压＋30kV，毛细管温度25℃，紫外检测波长205nm，压力进样5kPax10S。在此条件下，样品分析时间为2min，α-乳清蛋白和β-乳球蛋白的检出限（LOD）分别为3．0和12mg／L；迁移时间和峰面积的相对标准偏差（RSD，n=6）分别小于1％和6％，符合实际样品检测要求。本方法已成功用于实际乳糖样品的分析，在相关领域也有很好的应用前景。

英文摘要：

α-lactalbumin and fi-lactoglobulin are main impurities in the lactose products extracted from whey, which may cause allergy. Therefore, the separation and detection of race α-lactalbumin and β-lactoglobulin are of great importance to food and pharmaceutical industry. In this work, a simple, fast, sensitive and reproducible capillary zone electrophoresis （CZE） method has been developed for this purpose. After simple sample preparation procedure, α-lactalbumin and fl-lactoglobulin can be baseline separated within 2 minutes under following conditions ： 25 mM phosphate buffer at pH 7.0, ＋ 30 kV applied voltage, 25 ℃ capillary temperature,205 nm UV detection,and sample injection at 50 mbar for 10 so The limit of detection （LOD） at 3 times of S/N is 3.0 and 12 mg/L for α-lactalbumin andβ-actoglobulin, respectively. The repeatability （ RSD, n = 6） is below 1% for migration time and below 6% for peak area. These data qualified the method to the analysis of real lactose samples. The proposed method also shows a great potential in other fields of food and pharmaceutical industry.