为研究热带玉米光周期敏感性的遗传机制,利用温热组合黄早四×CML 288衍生的重组自交系群体构建了一个包含278个组合的永久F2群体,分别在海南三亚、河南郑州和洛阳、北京昌平和顺义等5个地点3种光周期环境中进行田间鉴定.利用复合区间作图法在3种光周期环境下共检测到16个不同的玉米叶片数相关QTL.位于第4染色体的QTL qLN 4-3在3个环境中同时检测到,表明该QTL在不同日照环境下能稳定表达;位于第10染色体上10.04区间的QTL qLN 10分别解释2个长日照环境下叶片数遗传变异的31.75%和39.96%,而在海南短日照环境中未检测到,表明该QTL是长日照环境下特异表达的主效叶片数QTL;位于第3染色体上的叶片数QTL qLN 3-1在短日照环境下能解释叶片数遗传变异的40.99%,而在2个长日照环境下并未检测到,表明此QTL是短日照环境下特异表达的主效QTL.
To study the genetic basis of photoperiod sensitivity in maize,animmortalized F2 population of 278 F2 cross was constructed by intercrossing recombinant inbred lines derived from a cross between temperate and tropical inbred lines(Huangzaosi×CML 288).The "immortalized F2" was evaluated for leaf number in five locations with three photoperiod environments,i.e.a short day environment of Sanya in Hainan Province,long-day environments of Zhengzhou and Luoyang in Henan Province,and long-day environments of Shunyi and Changping in Beijing.Sixteen QTLs for leaf number were detected in different photoperiod environments using composite interval mapping.The QTLs qLN 4-3 associated with leaf number were detected in all the three photoperiod environments,showing that this QTL might control leaf number steadily in different environments.The QTL qLN 10 in the bin 10.04 region of chromosome 10 associated with leaf number was detected in long day environments of Henan and Beijing and explained 31.75% and 39.96% of the phenotypic variation,respectively.This result indicated that the QTL qLN 10 might be a major leaf number QTL in long day environment.The QTL qLN 3-1 was detected for leaf number and explained 40.99% of the phenotypic variation in short day environment and not in long day environment,which indicated that the QTL qLN3-1 might control leaf number only in short day environment.