中文摘要：

目的探讨软骨细胞骨架的形态及含量变化在骨关节炎（OA）发病机制中的作用。方法 3月龄雄性新西兰白兔8只,随机分为2组,每组4只,实验组行前交叉韧带切断术（ACLT）,造成OA模型,对照组切开关节囊,但不行ACLT。20周后处死两组动物,取左膝做大体标本观察,行Mankin＇s评分及HE染色。右膝在无菌条件下取全层软骨,消化分离软骨细胞,荧光染色后用激光共聚焦扫描显微镜对软骨细胞骨架进行形态学观察及荧光强度定量检测,对数据进行两独立样本t检验和非参数检验。结果手术后20周实验组关节软骨明显退变,Mankin＇s评分明显高于对照组（t=7.23,P〈0.05）;和对照组相比,实验组波形蛋白、微管、纽蛋白形态及分布发生明显改变,荧光定量检测显示实验组中间纤维、微管和纽蛋白均低于对照组（t波形蛋白=5.40,t微管=11.86,t纽蛋白=5.21,P〈0.001）,微丝形态和含量两组间无统计学差异。结论 OA软骨细胞骨架中间纤维、微管和纽蛋白发生明显改变,可能与OA的发病机制有一定关系。

英文摘要：

ZHAO Hao-liang,LI Liang-liang,DUAN Wang-ping,LI Hao,WANG Xiao-hu,WEI Xiao-chun.Department of Orthopaedics,Second Hospital,Shanxi Medical University,Taiyuan 030001,China Objective To explore the effect of chondrocyte cytoskeleton changes both in morphology and in content,in terms of osteoarthritis（OA） pathogenesis.Methods Eight New Zealand white rabbits of three-month age,were divided into two groups randomly,four rabbits each.The animal model of OA was induced by anterior cruciate ligament transection（ACLT） in experimental group,and the joint capsule incision without ACLT was performed in control group.After 20 weeks,rabbits were sacrificed and evaluated by macroscopic,Mankin＇s score and HE staining in all left knees.Chondrocytes were isolated from cartilage of right knees in two groups.Fluorescent staining and laser scanning confocal microscope（LSCM） were used to examine the changes of chondrocytes cytoskeleton morphology and get quantitative determination of fluorescence intensity.All data were processed by Two-sample t-test and nonparametric test.Results The knee joints of experimental group had obvious degeneration of articular cartilage compared with control group.The macroscopic score of experimental group was significantly higher than control group（t = 7.23,P 0.05）.Intermediate filament,vinculine and microtubule in experiment group were aggregated and disrupted,and were significantly lower than control group according to fluorescent quantitative assay（tvimentin = 5.40,ttubulin = 11.86,tvinculin = 5.21,P 0.001）.Conclusions The pathogenesis of OA is related to the change of intermediate filament,microtubule and vinculin