研究了啤酒酵母的光散射光谱.结果表明,在波长308.0nm处,光散射强度与啤酒酵母浓度在2.0×10^4-2.0×10^6Cell/mL范围内呈线性关系,检出限(3σ)为4.94×10^2Cell/mL.将此方法成功地应用于培养液中啤酒酵母含量的快速、灵敏的测定,并对其散射光谱进行了表征.
The light scattering(LS) spectra of Saccharomyces cerevisiae was studied. The results show that the LS intensities were in proportion to the concentrations of Saccharomyces cerevisiae at peak 308.0 nm in the concentration range of 2. 0 × 10^4-2. 0 × 10^6 Cell/mL. The detection limit (3σ) was 4. 94 × 10^2 Cell/mL. The present new method was successfully applied to the rapid, sensitive determination and characterization of Saccharomyces cerevisiae biomass in suspensions.