中文摘要：

目的建立适于MRI研究的鼠脑胶质瘤模型。方法采用脑立体定向术，在Fischer344大鼠脑内接种F98胶质瘤细胞。接种后观察大鼠的生存期并进行生存分析。接种后第6、8、10、12、14天采用MRI及病理学方法动态观察肿瘤生长情况。结果接种成功率为100％，生存分析表明接种后老鼠多数于14d左右死亡。接种后6d，MRI即可检测出肿瘤生长。随着接种时间延长，肿瘤体积增大，接种后第6、8、10、12、14天组织学测量分别为（3．6±0．2）mm^3、（8．1±0．5）mm^3、（63．2±0．6）mm^3、（127．3±0．8）mm^3，（312．6±0．7）mm^3。MRI测量的肿瘤体积为（4．1±0．3）mm^2、（8．5±0．4）mm^3、（65．4±0．7）mm^3、（132．5±0．2）mm^3、（317．8±1．4）mm^3。组织学测量与MRI体积测量差异无统计学意义（t=0．943～2．287，P值均〉0．05），但两者明显相关（r=0．91，P〈0．01）。第10、12、14天组肿瘤体积较第6、8天组明显增大（F=3．52，P〈0．05）。病理学显示该肿瘤模型具有胶质瘤的病理特点。结论该胶质瘤模型成瘤率高，成瘤快，有良好的可预测性和可重复性，是适合MRI研究的理想模型。细胞接种后10～14d是最佳的研究时期。

英文摘要：

Objective To establish a rat model of glioma for MRI study. Methods Fischer 344 rats were inoculated with F98 glioma cells in caudate nucleus by stereotaxic procedure. MRI scanning and pathology examination were performed to observe the tumor growth on 6, 8, 10, 12, and 14 d, respectively, after inoculation and their survival was also analyzed. Results After inoculation, tumors were found in all rats by MRI as early as 6 days. Most of rats died on 14 d after inoculation. The volume of tumor increased as the inoculation time lengthened. The tumor volume measured by MRI was larger than actual size by autopsy, but the beth correlated positively （r = 0.91, P 〈 0.01 ）. The tumor had pathological features of glioma. Conclusion The animal model of glioma with high and rapid tumorigenesis is an ideal model for MRI study characterized by prospectability and repeatability, and its perfect study duration is between 10 and 14 days after inoculation of tumor cells.