以传统药用植物龙葵为实验材料,分离筛选能产生皂苷类活性成分的内生真菌。通过组织分离法,从龙葵的组织中分离得到24株内生菌。发酵实验结果表明,产皂苷类活性物质的菌株数共有8株,通过物理化学方法验证皂苷类物质主要存在于内生真菌的发酵液中。
Solanum nigrum L was used as materials to screen endophytic fungi which can produce saponins. 24 endophytic fungi were isolated from Solanum nigrum L by tissue surface - sterilized. The results indicated that 8 endophytic fungi can produce saponins, and they were mainly existing in the fermentation broth.