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中文摘要：
前期研究表明,铁原子对于嗜酸两性菌中硫氧化还原酶（SOR）的活性至关重要.本研究表明,2,2′-联吡啶、1,2-二羟基苯-3,5-二磺酸钠、8-羟基喹啉等特异性铁离子螯合剂强烈抑制腾冲嗜酸两性菌SOR酶活性,进一步表明铁原子是SOR酶活必需.对目前基因组数据库中的SOR基因或者SOR类似基因进行序列比对,发现了一个潜在的铁原子结合模体（H86-X3-H90-Xn-E114-Xn-E129）.据此,本研究采用定点突变技术,将氨基酸残基H86,H90和E129分别突变为苯丙氨酸或者丙氨酸,圆二色光谱测定发现突变体（H86F,H90F和E129A）的二级结构没有明显改变,但是这3个突变体全部丧失了酶活性.突变体蛋白中铁原子含量测定结果表明,3个突变体全部或者部分丢失了铁原子,而之前研究中获得的3个半胱氨酸突变体（完全丧失了酶活）铁原子含量没有变化.根据本研究并结合前期实验结果可知,SOR分子中模体C31-Xn-C101-X2-C104是底物硫分子活化区域;而模体H86-X3-H90-X23-E114-X14-（E/D）129是SOR分子中铁原子的结合区域,与铁原子结合形成一个非卟啉铁中心,是SOR的氧化还原中心;这两个区域均是SOR酶活性的必需部分.
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