中文摘要：

建立了基于硫脲和纳米金层层自组装技术的无标记、高灵敏电流型免疫传感器,用于萘的检测。利用循环伏安法研究了修饰电极表面的电化学特性以及测试溶液的pH值、孵育时间和温度对免疫传感器性能的影响。实验表明,此免疫传感器在含不同浓度萘抗原的PBS溶液（pH7.4）中37℃下孵育30 min后,在pH7.4的测试底液中测定,响应电流与萘浓度在0.5～100μg/L范围内有良好的线性关系,r=0.9986,检出限为0.08μg/L。此传感器制备简单,灵敏度高,稳定性好,可以重复使用。应用于实际水样中萘的测定,回收率为94.3%～107.0%。

英文摘要：

A new type of amperometric immunosensor for the determination of naphthalene based on self-assemble layer-by-layer technique was reported.The preparation procedure of such a sensor was characterized and the operation conditions,such as the pH value of supporting solution,incubated temperature and time were studied by cyclic voltammetry.Under the optimized working conditions,the current response of the immunosensor was proportional to the concentration of naphthalene in the range of 0.5-100 μg/L with a detection limit of 0.08 μg/L（r=0.9986）.This method was applied to the naphthalene detection in river water samples and the recovery was ranging in 94.3%-107.0%.The reported immunosensor exhibited high sensitivity and long-term stability for naphthalene detection,and was easy to be prepared and regenerated.