中文摘要：

对籼稻“成恢448”愈伤组织的诱导和分化过程进行了优化条件的探索．通过正交实验设计了不同的激素配比，结果显示：最佳的愈伤组织诱导条件为MS培养基加入1．0 mg/L 2，4-二氯苯氧乙酸（2，4-D），4．0 mg/L萘乙酸（NAA）和2．0 mg/L 激动素（ KT）；最佳的分化条件为 MS 培养基加入1．0 mg/L NAA，2．0 mg/L 6-苄氨基腺嘌呤（6-BA）和0．5 mg/L KT．

英文摘要：

Rice indica cultivar Cheng-hui 448, an excellent restorer line in hybrid rice , had resistant to the blast while sensitive to the bacterial blight .Recently, with transgenic technology , a number of resistant genes were applied in rice breeding .However , the tissue-culture systems of indica were still imperfect and signifi-cantly different in varieties .Here, optimization conditions of callus induction and differentiation for the indica variety Cheng-hui 448 was experimented by orthogonal test .According to the results , MS media with 1 .0 mg/L 2,4-D, 4.0 mg/L NAA and 2.0 mg/L KT, MS media with 1.0 mg/L NAA, 2.0 mg/L 6-BA and 0.5 mg/L KT, were the optimization conditions for callus induction and differentiation of Cheng-hui 448, respec-tively .