中文摘要：

目的：观察含右归丸鼠血清对人早孕流产胚胎脐带血管周围干细胞（FTM-PVCs）体外分化为类卵细胞的影响。方法：制备含右归丸鼠血清；取人早孕流产胚胎脐带组织分离纯化后加人不同浓度鼠血清诱导分化，设空白组、空白鼠血清组和人卵泡液组为对照；检测诱导分化后培养液中相关激素和细胞因子浓度、检测细胞诱导分化后卵细胞相关特异性标志物及其mRNA表达。结果：含右归丸鼠血清诱导人FTM-PVCs分化后，出现类卵细胞结构，表达GDF-9，减数分裂、卵细胞和透明带标志物mRNA表达升高（P〈0．05），培养液中E，、SCP2、MPF、F-T升高（P〈O．05）。结论：以人FTM-PVCs为研究平台，证实补肾中药能在体外诱导人FTM-PVCs向类卵细胞分化；含右归丸鼠血清能激活干细胞从休眠转为分化，说明温肾阳可唤醒肾精化气成形，机制可能与E，、SCP2、MPF、F．T相关。

英文摘要：

Objective： To investigate the effects of rat medicated sera prepared with Yougui Wan Formulae on differentiation of human first trimester-derived perivascular cells （FTM-PVCs） into oocyte-like ceils （OLCs） in vitro. Methods： The rats in blank group were gavaged with distilled water, the rats in Yougui Wan groups were gavaged with Yougui Wan. The rats sera were purified with HPLC and filtered to sterile. The human FTM-PV cells were first isolated and cultured according to the method published previously. The human FrM-PVCs were cultured with different percentages of the various rat medicated serum. At the same time, the cells were also treated with the rat blank serum as a negative control, while the human follicular fluid as a positive control. Concentrations of various cytokines and hormones in the rat sera as well as differentiation culture media with the rat sera were compared by using a number of specific ELISA kits, respectively. After interventions, the expression of GDF- 9 was examined with immunocytochemistry assay. For the group with 2% of the rat medicated serum, mR.NA levels of SCP3, GDF-9, ZP1, ZP2 and ZP3 were evaluated with a semi-quantitative RT-PCR assay. Results： The OLCs in morphology could be found in cultures with rat medicated serum that were similar to the positive controls while no significant changes in cell morphology was found in the negative controls. After the intervention with the rat medicated sera, the differentiated OLCs could express GDF- 9 protein, and mRNA levels of SCP3, GDF-9, ZP-1, ZP-2 and ZP-3 were significantly higher than that in the negative controls （P〈0.05）. E2, SCP2, MPF, F-T production in culture media with the rat medicated sera was significantly higher than that in culture media with the rat blank sera （P〈0.05）. Conclusion： Based on the research platform of Human FTM-PVCs, the rat medicated sera prepared with invigorating kidney herbs had the ability to induce human FTM-PVCs into OLCs in vitro. The rat medicated serum could activate the