中文摘要：

为探讨香青兰总黄酮在大鼠体内的药代动力学。采用以田蓟苷为指标,HPLC法测定大鼠灌胃给药后血浆中田蓟苷的浓度,并采用DAS2.0软件计算药代动力学参数。结果显示,血浆中田蓟苷浓度在0.031～39.68μg/mL范围内线性关系良好（R=0.9990）,日内精密度（RSD）〈5%,日间精密度（RSD）〈10%,方法回收率在98.01%～103.23%之间,提取回收率在72.67%～90.35%之间。香青兰总黄酮在大鼠体内呈二室分布,大鼠灌胃香青兰总黄酮提取物3个剂量（300,600,1200 mg/kg）后,t1/2β分别为（6.904±0.922）,（6.512±3.302）,（9.820±3.116）h,AUC（0-t）分别为（0.527±0.018）,（0.980±0.097）,（1.215±0.108）mg/L/h,Tmax分别为（0.292±0.083）,（0.139±0.048）,（0.222±0.048）h,Cmax分别为（0.177±0.018）,（（0.451±0.064）,（0.656±0.115）mg/L。由此可知,该法适用于测定香青兰总黄酮在大鼠体内的血药浓度并进行药代动力学研究。

英文摘要：

To study pharmacokinetics of Dracocephalum Moldavica L.Flavonoids in rat. Plasma concentration of tilianin was used as the indicators,which was measured by HPLC.The pharmacokinetic parameters were calculated by DAS2.0.It was showed good linear relationship when the concentration of tilianin in the range of 0.031 ～39.68 μg/mL with the correlation coefficient of 0.9990.The intra-day precision of this method was less than 5%,and the inter-day precision was less than 10%.The accuracy was 98.01%～103.23%.The extraction recovery was 72.67%～90.35%.The main pharmacokinetics parameters of tilianin after oral administration with three different doses（300,600,1200 mg/kg） to rats were： t1/2β：（6.904±0.922）,（6.512±3.302）,（9.820±3.116）h; AUC（0-t）：（0.527±0.018）,（0.980±0.097）,（1.215±0.108） mg/L/h;Tmax：（0.292±0.083）,（0.139±0.048）,（0.222±0.048）h;Cmax：（0.177±0.018）,（0.451 ±0.064）,（0.656 ±0.115） mg/L.The method is proved to be suitable for the pharmacokinetic study of Dracocephalum Moldavica L.Flavonoids in rats.