中文摘要：

以鸡β-防御素-1（Gallinacin-1，Gal-1）为研究对象，据GenBank中已发表的鸡β—actin和Gal-1序列设计引物，构建重组质粒作为标准品，成功建立了检测鸡免疫器官组织中Gal-1 mRNA表达的实时荧光定量PCR方法，并进行灵敏度、重复性、特异性试验。结果显示，此方法具有快速、高通量、线性范围广、特异性强、灵敏度高等特点，为进一步定量研究Gal-1mRNA表达及其与机体免疫机能的关系奠定了技术基础。

英文摘要：

The chicken beta-defensin-1 （Gallinacin-1, Gal-1） was regarded as the research object. According to the chicken β-actin and Gal-1 genes sequences published in GenBank, the recombinant plasmids were constructed for the standard, the study successfully established Real-time fluorescence quantitative PCR method for detecting the expression of Gal-1 mRNA in chicken, and tested the sensitivity, repetitiveness and specificity. The results showed that this method was characterized by rapidity, high-flux, wide linear range, good specificity, high sensitivity, and so on. So this method would provide the basis for making further efforts for quantitative research on the expression of Gal-1 mRNA and laying technical foundations for research on the related diseases.