通过研究褪黑激素主动免疫对生长猪生产性能和胴体性状的影响,探讨褪黑激素与生长猪养分沉积和肉质的关系。选用20kg左右健康二元杂交猪10头,按体重相近的原则随机分成2个处理,每个处理5头,分别注射牛血清白蛋白(BSA)和褪黑激素完全抗原,在试验第1天对试验猪进行首次主动免疫,免疫剂量为每只猪18mg,与福氏完全佐剂充分乳化后,在猪臀部多点肌肉注射。每14d加强免疫一次,剂量减半,与福氏不完全佐剂乳化后,在猪背部两侧对称多点皮下注射。加强免疫3次。结果表明,褪黑激素主动免疫有提高生长育肥猪生产性能的趋势;提高饲料蛋白质(2.15%)和能量(2.2%)消化率,降低饲料脂肪消化率;褪黑激素主动免疫能显著提高生长育肥猪眼肌面积(36.1%,P〈0.05),腹脂比显著增加(P〈0.05),但对背膘厚和屠宰率无显著影响(P〉0.05);各处理之间肉质指标无显著差异(P〉0.05),但褪黑激素主动免疫使滴水损失增加13.13%,大理石纹评分降低11.1%,肌肉丙二醛含量增加10.05%。褪黑激素主动免疫对生长育肥猪生产性能和胴体品质有一定改善作用,但对肉质有一定负面影响。
To evaluate the effects of active immunization against melatonin (MT) on performance and carcass quality of growing pigs and discuss the interaction between melatonin and carcass quality, ten Landrace x Large White growing pigs were allotted randomly to two groups by weight. Each group included five replicates with one pig. Pigs in each group were injected every 14 days with bovine serum albumin (BSA) or melatonin conjugated to BSA, respectively. The results showed that ADG, ADFI and FCR were not significantly different between the treatments(P〈0.05). The active immunization against melatonin increased the digestibility of feed protein and gross energy by 2.15% and 2.2%, respectively, and reduced the di- gestibility of ether extract. Treatment with MT increased logissimus muscle (LM) area by 36.1% (P〈0.05) and the rate of retroperitoneal fat was significantly increased ( P〈 0.05). There was no significant effect on backfat depth and rate of slaughter (RS) (P〉0.05). The difference of meat quality between groups was not significant(P〉0.05). Treatment with MT increased the content of maleic dialdehyde (MDA) in pork by 10.05% (P〉0.05). It is concluded that active immunization against melatonin has positive effects on performance and carcass quality, but negative effects on meat quality.