中文摘要：

根据从 orange-spottednervous 坏死病毒(OGNNV ) 的染色体的顺序和分析，中国拉紧，一双特殊教材从 OGNNV 根据 RNA2 的核苷酸序列被设计。OGNNV 的主要衣壳蛋白质(MCP ) 基因借助于 reverse-transcriptase 聚合酶链反应(RT-PCR ) 被克隆并且绑扎进 pET32aexpression 原生质标志。OGNNV 的 MCP 基因是 1 017 个底，与 37.1 kDa 的一个分子的团编码了 338 氨酸的蛋白质。有 57.4 kDa 的一个分子的团的 Recombinant 蛋白质是表示 inE。关口 i BL21 (DE3 ) 。疫苗从在 recombinantcells 表示的 recombinant 蛋白质被准备。少年看到橘子的组 ers (在平均长度的 8 厘米) 被使免疫 byintraperitoneal 注射。组 A 与感染的织物被质问过滤 25 dpost 种痘。在 vaccined 的死亡组织(A1， 30%) 比 theunvaccined 组高一些(B2， 27.8%) 。组 B 在三疫苗的注射以后被质问。在 vaccined 组的死亡(B1， 16.7%) 比 unvaccined 组低(B2， 27。8%) ，并且与 unvaccined 组相比， vaccined 组的相对百分比幸存(RPS ) 价值是 40% 。Theanti-recombinant 蛋白质重量的单位一 with 1:100 冲淡与感染的织物的两倍体积被混合过滤并且为 12 h 在 4 点孵化了℃然后肌内地注入了看到 juvenileorange 的 grouper。感染的组织的治疗与 anti-recombinant 过滤蛋白质浆液导致了鱼的显著地更低的死亡(组 C1， 18.18% 的死亡) ，与鱼相比(组 C2， 40% 的死亡) 它收到了感染的组织过滤与控制对待浆液。结果对 OGNNV 在免疫暗示了衣壳蛋白质的潜在的使用。

英文摘要：

On the basis of the sequence and analysis of genome from the orange-spotted nervous necrosis virus（ OGNNV）, China strain, a pair of special primers were designed according to the nucleotide sequences of RNA2 from OGNNV. The major capsid protein （ MCP）gene of OGNNV was cloned by means of reverse-transcriptase polymerase chain reaction （RT-PCR） and ligated into the pET32a expression plasmid. The MCP gene of OGNNV was 1 017 bases, encoded a protein of 338 amino acid with a molecular mass of 37.1 kDa. Recombinant protein with a molecular mass of 57.4 kDa was expressed in E. coli BL21 （DE3）. Vaccine was prepared from the recombinant protein expressed in recombinant cells. The juvenile orange-spotted groupers （8 cm in average length） were immunized by intraperitoneal injection. Group A was challenged with infected tissue filtrates 25 d post-vaccination. The mortality in the vaccined group （ A1,30% ） was a little higher than the unvaccined group （ B2, 27.8% ）. Group B was challenged after three vaccine injections. The mortality in the vaccined group （B1, 16.7% ） was lower than the unvaccined group （132, 27.8% ）, And the relative percentage survival （RPS） value of vaccined group, compared with the unvaccined group, was 40%. The anti-recombinant protein sera with a 1 ： 100 dilution were mixed with double volume of infected tissue filtrates and incubated at 4 ℃ for 12 h and then intramuscularly injected into the juvenile orange-spotted grouper. Treatment of infected tissue filtrates with anti-recombinant protein serum resulted in a significantly lower mortality of fish （ Group C1, mortality of 18.18% ）, compared with the fish （ Group C2, mortality of 40% ） which received infected tissue filtrates treated with control serum. Results implied the potential use of the capsid protein in immunization against OGNNV.