为了解生态恢复过程中土壤微生物功能特征,采用Biolog-Eco微平板法分析了黄土丘陵区人工灌木(柠条Caragana korshinskii、沙棘Hippophaer rhamnoides)、人工草地(沙打旺Astragalus adsurgens、柳枝稷Panicum uirgatum)和天然草地(阿尔泰狗哇花Heteropappus altaicus、茵陈蒿Artemisia capillaries)的根际微生物碳源代谢多样性特征.结果表明:柠条、阿尔泰狗哇花和茵陈蒿的根际微生物对糖类的代谢能力显著高于沙棘、沙打旺和柳枝稷;阿尔泰狗哇花和茵陈蒿对氨基酸类、酚酸类和聚合物类的代谢能力较高。茵陈蒿和阿尔泰狗哇花的微生物代谢功能多样性高于其他4种植物.柠条、阿尔泰狗哇花和茵陈蒿具有相似的根际微生物代谢功能。黄土丘陵区植被类型显著影响微生物群落碳源利用能力,天然草地根际微生物群落代谢功能多样性优于人工灌木和人工草地。
To explore the metabolic function of soil microbial community in the ecological restoration,the rhizosphere microbial carbon utilization diversity of artificial shrubland {Caragana korshinskii,Hippophaer rhamnoides),artificial grassland(Astragalus adsurgens,Panicum virgatum),and natural grassland(Heteropappus altaicus,Artemisia capillaries) were analyzed on the abandoned cropland of hillygully region of Loess Plateau using Biolog-Eco microplate method.The results showed that the capabilities of soil microbes in metabolizing carbohydrates were significantly higher in the rhizsophere of Caragana korshinskii,Heteropappus altaicus,and Artemisia capillaries than that in the Hippophaer rhamnoides,Astragalus adsurgens,and Panicum virgatum.Among the six plants,Heteropappus altaicus and Artemisia capillaries presented the higher capabilities of metabolizing amino acids,phenolic acids and polymers.The functional diversities of microbial community were higher in the rhizosphere of Heteropappus altaicus and Artemisia capillaries than that in the other test plants.Caragana korshinskii,Heteropappus altaicus and Artemisia capillaries had the similar microbial metabolic function.Vegetation types had a significant effect on the carbon utilization of rhizosphere microbial community in the abandoned cropland of hilly-gully region of Loess Plateau.Natural grassland had the more functional diversity of microbial metabolism than the artificial shrubland and artificial grassland.