中文摘要：

目的研究ClC-3反义寡核苷酸对H2O2诱导的大鼠主动脉平滑肌细胞凋亡的影响。方法蛋白免疫印迹法检测ClC-3蛋白表达；形态学方法、DNA琼脂糖电泳、MTT法和流式细胞仪观察和分析H2O2诱导的大鼠主动脉平滑肌细胞形态学改变、DNA断裂、细胞存活率和凋亡率及ClC-3反义寡核苷酸转染对其影响。结果ClC-3反义寡核苷酸转染抑制内源性ClG3蛋白表达后，可加重H2O2诱导大鼠主动脉平滑肌细胞形态学改变及DNA断裂，细胞凋亡率由52．8％±13．6％增至75．7％±5．8％（n=6，P〈0．01），而细胞存活率由48．9％±4．3％进一步降低为31．3％±4．3％（n=6，P〈0．01）。结论ClC-3反义寡核苷酸转染促进H2O2诱导的大鼠主动脉平滑肌细胞凋亡。

英文摘要：

Aim To investigate the effects of ClC-3 antisense oligonucleotide on H2O2 induced apoptosis in rat aortic vascular smooth muscle cells. Methods Western-blot was performed to detect the expression of ClC-3 protein. Morphological determination, DNA agarose gel electrophoresis, MTT assay and flow cytometry analysis were performed to measure the morphological changes, DNA ladder, viability and apoplotic rate and effects of ClC-3 antisense oligonucleotide on rat aortic smooth muscle cells. Results The antisense oligonucleotide specific to ClC-3 mRNA decreased the expression of ClC-3 protein and enhanced the apoptotic events induced by H2O2 including ultrastructural alteration and DNA ladder. The apoptotic cell death rate increased from 52.8% ± 13.6% to 75.7% ±5.8% （n = 6, P 〈 0.01）,and whereas the viability further decreased from48.9% ±4.3% to 31.3%±4.3 % （n=6,P〈0.01） by ClC-3 anfisonse oligonueleotide transfection. Condusion ClC-3 antisense oligonucleotide potentiated apoplosis induced by H2O2 in rat aortic smooth muscle cells.