中文摘要：

目的研究莫诺苷对缺血再灌注7 d大鼠皮层中金属基质蛋白酶组织抑制剂（tissue inhibitor of metalloproteinase,TIMP）表达的影响。方法 25只雄性SD大鼠采用改良Zealonga线栓法制备大鼠大脑中动脉阻塞（middle cerebral artery occlusion,MCAO）模型,术后随机分为模型组、莫诺苷小剂量组、中剂量组、大剂量组,每组5只。造模3 h后按30、90、270 mg/kg剂量每天一次灌胃莫诺苷。免疫印迹法（Western Blotting）和免疫荧光染色法（Immunofluorencent staining）分析莫诺苷对脑缺血再灌注7 d大鼠患侧皮层TIMP表达的影响。结果脑缺血再灌注7 d后,与假手术组相比,模型组TIMP蛋白表达显著升高（P〈0.01）;同模型组相比,莫诺苷中剂量组（90 mg/kg）和大剂量组（270 mg/kg）TIMP蛋白表达均明显升高（P〈0.05;P〈0.01）。结论莫诺苷可以上调TIMP在大脑皮层内的表达,有助于脑卒中后血脑屏障的保护及修复。

英文摘要：

Objective To study the effects of morroniside on the expression of the tissue inhibitor of metalloproteinase（ TIMP） of the ischemic ipsilateral cortex 7 days after ischemia reperfusion. Methods 25 male SpragueDawley rats were subjected to middle cerebral artery occlusion（ MCAO） model with modified Zea Longa＇s method,and then divided them into sham group（ n = 5）,ischmia group（ n = 5）,and morroniside groups（ low,medium,and high dosage groups,n = 5） randomly. Morroniside were then administered intragastrically once a day at dose of 30 mg / kg,90 mg / kg and 270 mg / kg 3 hours after operation. The expression of TIMP of the ischemic ipsilateral cortex on 7 days after ischemia-reperfusion were detected by western blotting and immunofluorescent staining analysis. Results Compared with the sham group,the expression of TIMP in ischemia group increased（ P 〈0. 05） 7 days after MCAO. Compared with the ischemia group,after treatment with morroniside at doses of 90 mg / kg and 270 mg / kg,the expression of TIMP increased significantly（ P 〈0. 05; P 〈0. 01）. Conclusions Morroniside could increase the expression of TIMP in the ischemic ipsilateral cortex and may contribute to blood-brain barrier protection and restoration.