中文摘要：

目的：观察唐古特大黄多糖（rheum tanguticum polysaccharides，RTP）对小鼠溃疡性结肠炎（ulcerative colitis，UC）的疗效并探讨其可能存在的机制。方法：应用三硝基苯磺酸（TNBS）灌肠制备小鼠溃疡性结肠炎模型。实验设正常对照组、模型对照组、RTP治疗组（100、200、400mg·kg^-1）。观察各组实验小鼠体质量变化及组织病理学改变，采用酶联免疫吸附法检测细胞因子（IL-8、IL-10、TNF-α、IL-4）的含量。结果：中、高剂量的RTP能够缓解小鼠体质量的减轻，减少腹泻及便血的发生；并且能够显著改善小鼠结肠组织损伤及病理学改变。渍疡性结肠炎模型组TNF-α、IL-8的表达较正常对照组显著增高，而IL-10、IL-4的表达明显降低（P〈0．05）；高剂量RTP治疗组的TNF-α、IL-8表达明显低于溃疡性结肠炎模型组，IL-10、IL-4的表达显著高于模型组（P〈0．01）。结论：RTP对TNBS诱导的小鼠溃疡性结肠炎具有良好治疗作用，其机制可能与上调抗炎细胞因子（IL-10、IL-4）及下调促炎细胞因子有关。

英文摘要：

AIM： To explore the effects of rheum tanguticum polysaccharides （RTP） on TNBS -induced colitis in mice and its probable mechanisms. METHODS： Mice colitis model was induced by 2, 4, 6-trinitrobenzene sulfonic acid （TNBS）. The mice were randomly divided into 5 groups,normal group, model group, RTP-100, 200 and 400 group. The maeroseopieal and histological changes of the colon were evaluated and the eytokines IL-8, IL- 10, TNF-α and IL-4 produced by splenoeyts were analyzed with ELISA. RESULTS： Compared with the model group, both symptoms and the lesions of colonic mueosa of RTP-200 and 400 group were slighter on ulcerative coliris induced by TNBS in mice. Furthermore, the expressions of TNF-aand IL-8 in colon tissue of mice with TN- BS-induced colitis were higher and the IL-10 and IL-4 were lower than that of normal control （ P 〈 0.05 ）. In RTP-400 group, the positive expressions of TNF-α and IL-8 were lower and IL-10 and IL-4 were higher than that of model group（ P 〈 0.01 ）. CONCLUSION： RTP has significant effects on TNBS-induced colitis in mice, the mechanism of which may relate to its regulation on cytokines.