中文摘要：

目的利用响应面法对桑黄菌液体发酵培养基进行优化,提高胞外多糖产量。方法应用Plackett-Burman设计和响应面法,对桑黄菌液体发酵培养基成分及其含量进行优化。用Design-Expert软件对实验数据进行多元回归分析,并建立3种主要因素（蛋白胨、酵母粉和磷酸二氢钾）与多糖产量之间的函数关系。用最终优化的配方进行3次验证实验。结果通过快速＂登高法＂对3个显著因素进行寻优,培养基中3因素最佳浓度为：蛋白胨14.72 g/L、酵母粉11.04 g/L、磷酸二氢钾1.472 g/L,胞外多糖产量最高可达7.04 g/L。3次验证实验所测得的多糖产量分别为6.92、6.86和7.04 g/L,平均值为6.94 g/L,与预测结果基本一致。结论优化的桑黄菌发酵培养基可显著提高胞外多糖产量。

英文摘要：

Objective To optimize the medium for fermentation culture of Phellinus igniarius by response surface method and increase the yield of exopolysaccharide.Methods The primary components of liquid medium for P.igniarius was optimized by Plackett-Burman design and response surface method.The experimental data were subjected to polynomial regression analysis by Design-Expert software,based on which the functional relationship between the three primary components（peptone,yeast extract and potassium dihydrogen phosphate） and the yield of exopolysaccharide was explored.Three check tests were performed on the optimized medium.Results The concentrations of peptone,yeast extract and potassium dihydrogen phosphate were optimized as 14.72,11.04 and 1.472 g/L respectively by ridge analysis.By using the optimized medium,the highest yield of exopolysaccharide reached 7.04 g/L.The yields of exopolysaccharide in three check tests were 6.92,6.86 and 7.04 g/L respectively,with a mean of 6.94 g/L,which were basically consistent with those expected.Conclusion The yield of exopolysaccharide increased significantly by using the optmizied medium.