中文摘要：

【目的】研究不同根瘤菌株对豌豆生长发育、产量及品质的影响,并分析比较不同根瘤菌株的竞争结瘤能力,筛选出与供试豌豆品种匹配较好的根瘤菌株。【方法】在盆栽条件下,应用湖南省华容县棉田低氮土壤,对加拿大半无叶型豌豆MISER分别接种9株豌豆根瘤菌（CCBAU 33109、CCBAU 43228、CCBAU 43227、CCBAU 43232、ACCC 16058、CCBAU 41242、CCBAU 43242及商用菌株F98-1和F98-4）。测定豌豆的生育时期;在豌豆盛花期测定豌豆的主要生育特征、根瘤菌的结瘤固氮性能,并用BOX-PCR指纹图谱技术测定根瘤菌的占瘤率;在成熟期测定豌豆的主要生育特征、产量、产量构成因子,及其干籽粒粗蛋白、粗纤维含量。【结果】与不接种对照（CK）相比,接种CCBAU 33109后,MISER盛荚期和成熟期分别延长4.1和7 d（P〈0.05,下同）;接种ACCC 16058后,主茎节数在成熟期分别比对照、F98-1和F98-4多2.8、3.8和2.9节,株高比F98-4高15.67%,粗蛋白含量比对照和F98-1分别提高9.88%和10.63%;接种CCBAU 41242后,豌豆单株籽粒鲜重比商用菌株F98-1提高40.46%;接种菌株CCBAU 43228的占瘤率最高,为57.50%。【结论】根据接种根瘤菌对豌豆生长发育、产量以及品质等方面的影响,结合各根瘤菌株的竞争结瘤能力,本研究筛选出较适于豌豆MISER的豌豆根瘤菌株CCBAU 33109、ACCC 16058、CCBAU 41242、CCBAU 43228。

英文摘要：

[Objective] The objective of this study is to determine the effects of inoculating pea with different Rhizobium leguminosarum strains on pea＇s growth and development, yield, grain quality, the competitive nodulation ability of the inoculated strains, and rhizobium strains best matching with the pea variety. [Method] A pot experiment was conducted by inoculating a Canadian semi-leafless pea variety ＇MISER＇ with 9 R. leguminosarum strains （CCBAU 33109, CCBAU 43228, CCBAU 43227, CCBAU 43232, ACCC 16058, CCBAU 41242, CCBAU 43242 and 2 commercial rhizobia F98-1, F98-4） in low nitrogen soil from a cotton field in Huarong County, Hunan Province. Growing stages of pea were investigated. Pea＇s main growth characteristics, nodulation and nitrogen fixation performance were measured at full-fowefing stage, and meanwhile, the nodule occupancy of eachinoculated strain was assayed using BOX-PCR fingerprinting technology. Pea＇s main growth characteristics, yield, yield components, crude protein and crude fiber content of dry grains were determined at maturing stage. ~ Result] Compared with not-inoculated treatment （CK）, the podding and pod ripening periods of pea inoculated with CCBAU 33109 were significantly increased by 4.1 and 7 d （P〈0.05）, respectively. At pod ripening period, the numbers of main stem nodes of pea inoculated with ACCC 16058 were significantly increased by 2.8, 3.8 and 2.9, compared with CK and treatments inoculated with F98-1 and F98-4, respectively. The height of pea inoculated with ACCC 16058 increased significantly by 15.67% compared with F98-4. Compared with CK and the treatment inoculated with F98-1, inoculating with ACCC 16058 significantly increased MISER＇s crude protein content by 9.88% and 10.63%, respectively. The grain fresh weight per plant of peas inoculated with CCBAU 41242 was also significantly 40.46% heavier than that of peas inoculated with F98-1. The nodule occupancy of CCBAU 43228 was 57.50%, higher than that of any other strain. [Conclusion] Based on the analys