中文摘要：

目的：探讨黄连总生物碱对糖尿病大鼠的降血糖作用。方法：采用链脲佐菌素50 mg/kg诱导SD大鼠形成糖尿病模型,筛选糖尿病大鼠空腹血糖的禁食时间,考察黄连总生物碱给药68d对糖尿病大鼠血糖、糖化血清蛋白、肌酐、尿素氮的影响,并测定其肝、胃、肾脏器指数,评价黄连总生物碱对糖尿病大鼠的降血糖作用。结果：（1）选择白天禁食6 h为糖尿病大鼠的禁食时间。（2）与模型组比较,黄连总生物碱高剂量组（含黄连总生物碱578.7 mg/kg）可明显降低糖尿病大鼠空腹6 h血糖及糖化血清蛋白（P〈0.05）;黄连总生物碱低剂量组（含黄连总生物碱192.9 mg/kg）可显著降低谷胱甘肽-过氧化物酶（P〈0.01）。黄连总生物碱对糖尿病大鼠肌酐、超氧化物歧化酶、总胆固醇、甘油三酯及脏器指数总体上影响不明显（P〉0.05）。结论：黄连总生物碱对糖尿病大鼠有降血糖的作用。

英文摘要：

AIM：To study the hypoglycemic effect of total alkaloids from Rhizoma Coptidis in streptozotocin diabetes rats. METHODS：The diabetic rats model was established by single intraperitoneal injection of streptozotocin 50 mg/kg.The fasting time of diabetic rat model was screened.Sprague-Dawley rats had been successively administrated total alkaloids from Rhizoma Coptidis（578.7 and 192.9 mg/kg） for 68 days.The levels of fasting blood glucose,glycosylated serum protein,serum creatinine and urea nitrogen were assayed.The liver,spleen and kidney organ indexes were measured.The hypoglycemic effect of total alkaloids from Rhizoma Coptidis in streptozotocin diabetes rats was evaluated.RESULTS：（1）Diabetic rats could be chosen to fast 6 h during the day for measuring fasting blood glucose.（2） Compared with diabetic model group,the group of total alkaloids from Rhizoma Coptidis 578.7 mg/kg could significantly decrease the 6 h fasting blood glucose and glycosylated serum protein（P0.05） and the group of total alkaloids from Rhizoma Coptidis 192.9 mg/kg could significantly reduce the level of glutathione peroxidase（P0.01）.Total alkaloids from Rhizoma Coptidis had no impact on serum creatinine,superoxide dismutase,total cholesterol,triglyceride and liver,spleen,kidney organ indexes of diabetes rats（P0.05）.CONCLUSION：Total alkaloids from Rhizoma Coptidis have hypoglycemic effect in streptozotocin diabetes rats.