以制浆厂麦草备料废渣为原料,探讨了稀硫酸水解预处理对其主要化学成分及酶水解糖化效率的影响。结果表明:麦草备料废渣经170℃自水解预处理后,酶水解效率较低;预处理中加入不同量的稀硫酸水解有利于促进麦草备料废渣的酶水解糖化。未经洗涤直接磨浆得到的预处理浆料的酶水解总糖得率高于经水洗后的浆料。预处理后物料不经洗涤直接酶水解的总糖转化率随预处理酸用量的增加呈先升后降的趋势,经稀硫酸水解(H2SO4质量分数0.32%,170℃)和PFI磨浆(8000 r)预处理得到的麦草备料废渣浆料,未经洗涤在酶用量20μmol/( min·g)下酶水解,其葡聚糖和木聚糖转化率分别为71.9%和73.9%,总糖转化率达到最大值(近70%)。
The wheat straw handling residue produced in pulp mills are difficult to be utilized efficiently and economically due to the complicated composition and large quantities. However, as a cheap lignocellulosic material, the utilization of the residue to produce fuel ethanol offers a great potential for reducing solid waste emission and producing great economic benefits. The effects of dilute acid hydrolysis pretreatment on chemical composition and enzymatic saccharification of wheat straw handling residue were investigated. The results showed that the sugar conversion of enzymatic saccharification was low after autohydrolysis pretreatment at 170℃. The addition of dilute sulfuric acid in the pretreatment was beneficial to enhance the enzymatic saccharification. The sugar conversion of unwashed pretreated wheat straw handling residue was higher than that of washed one. The total sugar conversion of unwashed pretreated materials increased with the increase dosage of sulfuric acid, and then it decreased with the continuous increasing charge. The glucan and xylan conversion reached up to 71.9% and 73.9%, respectively, and the total sugar conversion reached the maximum ( closed to 70%), for the substrate with prehydrolysis (0.32% H2SO4, 170℃) and PFI refining (8 000 revolutions), hydrolyzed at a cellulase loading of 20 μmol/( min·g) .