中文摘要：

目的：研究核因子κB抑制蛋白（IκBα）在急性胰腺炎（AP）大鼠胰腺和肝脏组织中的表达及中药新清胰汤Ⅱ号[Qing Yi TangⅡ（QYT）]的影响。方法：70只SD大鼠随机被分为正常对照组（n=10）、AP＋QYT组（n=30）、AP＋生理盐水（NS）组（n=30）。经胰胆管逆行注射4％去氧胆酸钠复制AP模型；给予QYT（AP＋QYT组）或NS（AP＋NS组）灌胃，每5h重复1次。造模后1h、4h和10h分批处死动物。荧光定量RT—PCR、Western blotting分别检测胰腺和肝脏组织IκBα在mRNA水平和蛋白水平的表达，后者包括磷酸化形式和非磷酸化形式；ELISA法检测血清白三烯C4（LTC4）浓度；HE染色观察胰腺和肺组织病理学变化。结果：AP大鼠肝脏组织中IκBα mRNA表达在各个时点均显著高于正常对照组（P〈0．01，P〈0、05）；AP＋QYT组显著低于AP＋NS组（P〈0．05）。与IκBα的mRNA表达相一致，肝脏和胰腺组织中IκBα蛋白表达（无论其磷酸化形式和非磷酸化形式）在AP大鼠均高于正常对照组（P〈0、05）；AP＋NS组IκBα蛋白磷酸化形式表达在观测时间内增高；QYT可抑制该形式的表达（P〈0．05）。AP大鼠血清LTC4浓度明显高于正常对照组，呈时间依赖性；AP＋QYT组血清LTC4浓度明显低于AP组（P〈0．05）。病理学检查见AP大鼠胰腺组织水肿、出血、肺间质水肿、出血，炎症细胞大量浸润，随AP的病程而加重；QYT治疗组的病理变化较轻。结论：QYT可通过抑制IκBα的磷酸化等机制减轻AP时局部和全身的炎症反应。

英文摘要：

AIM： To investigate the expression of inhibitor kappa B alpha （IκBα） in panereas and liver tissues of rats with experimental aeute panereatitis （AP） and to explore the therapeutie mechanism of Chinese medicine new Qing Yi Tang （QYT） on AP. METHODS： 70 SD rats were randomly divided into three groups： normal eontrol group （n = 10 ） , AP ＋ QYT group （ n = 30） , and AP ＋ normal saline （NS） group （ n = 30 ）. AP model was induced by retrograded injeetion of 4% sodium deoxyeholate into the pancreatic duet. QYT or NS was infused to the rats respectively by a gastric catheter repeatedly every five hours after AP induetion. At 1 h, 4 h, 10 h after operation, rats were saerifieed, and the panereas and liver were moved out individually. Real - time RT - PCR was performed to deteet IκBα mRNA expression in the liver. Western blotting was applied to detect IκBα protein expression in the liver and pancreas. IκBα proteins including phosphorylated form （ IκBα - p） and non - phosphorylated form （ IκBα - n） were tested. Serum level of leukotriene C4 （ LTC4 ） was deteeted by ELISA. The pathologieal ehanges of panereas and lung tissues stained with HE were observed under light mieroseope. RESULTS： Compared with the normal eontrol group, expression of IκBα mRNA in liver was higher in AP rats in the observation period （P 〈0. 01, or P 〈 0.05 ）. QYT treated group had lower expression of IκBα mRNA as eompared with AP ＋ NS group （ P 〈0.05 ）. Expression of IκBα protein （ ineluding IκBα - p and IκBα - n） in liver and panereas were also higher in AP group as eompared with that in eontrol group （P 〈 0. 05 ）. IκBα -p displayed an inereased tendeney during the observation period in AP ＋ NS group. However, QYT treatment induced a decrease in IκBα -p protein expression and an increase in IκBα - n expression. The serum LTC4 level in AP group was inereased in a time - dependent manner, and QYT attenuated the increased LTC4 level in certain deg