中文摘要：

目的观察补肾安胎冲剂对复发性自然流产（recurrent spontaneous abortion，RSA）小鼠蜕膜组织血管、细胞形态及血管内皮细胞超微结构的影响。方法采用DBA／2×CBA小鼠，按照Clark经典方法复制RSA小鼠模型。按妊娠顺序将小鼠随机分为正常妊娠组，模型组，西药组，补肾安胎冲剂高、中、低剂量组，每组10只，分别进行药物干预。光镜下观察蜕膜组织的血管和细胞形态，电镜下观察血管内皮细胞超微结构。结果光镜下见模型组血管数量减少，蜕膜细胞胞浆明显水肿，部分核消失，细胞核固缩；补肾安胎冲剂高剂量组血管丰富且管壁完整，由多个血管内皮细胞环绕而成，小部分蜕膜细胞水肿，细胞核基本无坏死；补肾安胎冲剂中、低剂量组血管数量少于补肾安胎冲剂高剂量组，血管内皮细胞排列不整齐，部分蜕膜细胞胞浆水肿，空泡变，少数细胞核消失。电镜下见模型组血管壁不完整，血管内皮细胞结构不完整，细胞核呈梭形，粗面内质网、核糖体减少，部分线粒体已破坏；补肾安胎冲剂高剂量组血管壁完整，血管内皮细胞结构完整，核膜完整；胞浆中可见丰富粗面内质网及核糖体，线粒体结构完整、无肿胀变性，染色质分布均匀；补肾安胎冲剂中、低剂量组血管壁完整，部分血管内皮细胞肿胀，核膜完整，胞浆中粗面内质网扩张，核糖体轻度减少，部分线粒体肿胀、空泡变。结论RSA模型小鼠存在母胎界面血管生成的障碍，补肾安胎冲剂可以修复RSA小鼠蜕膜组织中破坏的细胞结构，改善血管生成和细胞器完整度，发挥安胎作用。

英文摘要：

Objective To investigate the effects of granules for tonifying the kidney and preventing miscarriage （GTKPM） on the blood vessels, cell morphology, and ultrastructure of vascular endothelial cells （VECs） of the decidual tissue in mice with recurrent spontaneous abortion （RSA）. Methods DBA/2× CBA mice were used to induce the mouse model of RSA by the classic Clark method. A total of 50 RSA model mice （CBA/J× DBA/2） were prepared, and 10 normal pregnancy mice （CBA/J × BALB/c） were prepared. The RSA model mice were randomly divided into model group, Western medicine group, high-dose GTKPM group, middle-dose GTKPM group, and low-dose GTKPM group （n=10 for each group）. And they received respective interventions. The changes in blood vessels and cell morphology of decidual tissue were observed under a light microscope, and the changes in ultrastructure of VECs were observed under an electron microscope. Results Under the light microscope, reduced number of blood vessels, obvious decidual cell cytoplasm edema, partial nuclear disappearance, and nuclear pyknosis in some decidual cells were observed in the model group; in the high-dose GTKPM group, the rich blood vessels were composed of many VECs and the vascular wall was integral, edema appeared in some decidual cells, and there were few necrotic nuclei; the middle- and low-dose GTKPM groups had few blood vessels than the high- dose GTKPM group, and in the two groups VECs were in disordered arrangement, edema and vacuolization of cytoplasm appeared in some decidual cells, and some nuclei disappeared. Under the electron micro-scope, in the model group, the vascular wall was incomplete, the structure of VECs was incomplete, nuclei were spindle-shaped, rough endoplasmic retieulums and ribosomes decreased, and some mitochondria were destroyed; in the high-dose GTKPM group, the vascular wall was integral, the structure of VECs and nuclear membrane were complete, there were rich rough endoplasmic reticulums and ribosomes, the structure of mitoehondr