中文摘要：

[目的]研究铜对涡虫运动和再生的影响。[方法]用不同浓度的铜溶液处理涡虫,分别进行了4和6d的培养试验,并设置空白对照组。[结果]成体涡虫24,48,72和96h运动的半抑制浓度（mIC50）分别为3.86,3.73,3.47和2.61mgCu2＋/L。幼虫24,48,72和96h运动的半抑制浓度（mIC50）分别为2.22,1.64,0.87和0.93mgCu2＋/L。整体呈现出浓度效应关系。涡虫6d的眼点和耳突再生的半抑制浓度分别是0.76,0.78mgCu2＋/L。呈现出浓度效应关系。[结论]支持涡虫作为水生生态系统的指示生物。

英文摘要：

[Objective] A mobility measurement and a regeneration assay were conducted to explore the behavioral effects of Cu2＋ on planarian Dugesia japonica. [Method] Dugesia japonica was respectively treated with different concentrations of copper solution for 4 and 6 d, and the blank control group was set. The mobility measurement and a regeneration assay of copper ions were determined by mIC50 and rIC50 . [Result] The mobility IC 50 for adult D. japonica at 24, 48, 72 and 96 h were 86, 3.73, 3.47 and 2.61 mg Cu2＋ /L, respectively. The mobility IC50s for newborns was 2.22, 1.64, 0.87 and 0.93 mg Cu2＋ /L, respectively. Although there was no significant difference between 24 and 48 h, and between 72 and 96 h of copper exposure, Cu2＋ decreased newborns locomotor behavior in a concentration-dependent manner at sub-toxic concentrations. The regeneration IC50 of eyespots and auricles for adult D. japonica at 6 d were calculated as 0.76, 0.78 mg Cu2＋ /L, respectively. The number of eyespots and auricles regeneration in planarian D. japonica showed a concentration-dependent manner. Results showed that newborns mobility and adult regeneration assay for D. japonica in a concentration-dependent manner. [Conclusion] The results of the present study suggest that planarians can be regarded as a useful bioindicator species for toxicological studies in the future.