中文摘要：

目的：探讨低剂量氢醌（HQ）对大鼠骨髓间充质干细胞（BMSCs）生物学性状及聚腺苷二磷酸核糖聚合酶-1（PARP-1）基因表达的影响,阐明低剂量HQ对骨髓的毒效应。方法：磷酸盐缓冲液（PBS）溶解HQ,以PBS处理组为对照组,分别以2.5、5.0和10.0μmol·L-1HQ染毒大鼠BMSCs为处理组。应用Cell Counting Kit-8试剂盒检测细胞活力和细胞增殖能力,通过磷脂结合蛋白（Annexin V）/碘化丙啶（PI）标记的流式细胞技术检测细胞凋亡,用实时荧光定量-聚合酶链反应（qRT-PCR）检测PARP-1基因表达的改变。结果：与PBS对照组比较,各剂量组细胞增殖能力明显提高（P〈0.05）;染毒48 h后,5.0和10.0μmol·L-1HQ组细胞的凋亡率均明显降低（P〈0.05）;染毒48 h后,2.5、5.0和10.0μmol·L-1HQ组细胞PARP-1表达量分别为对照组0.92、0.56（P〈0.05）和0.45倍（P〈0.05）。结论：低剂量HQ能抑制大鼠BMSCs凋亡和PARP-1基因表达,促进细胞增殖。

英文摘要：

Objective To explore the influences of low level hydroquinone（HQ） in biological characteristics and the expression of poly（ADP-ribose） polymerase1（PARP-1）gene in rat bone mesenchymal stem cells（BMSCs）,and elucidate the toxic effect of low dose HQ on bone marrow.Methods HQ dissolved in PBS buffer at the concentrations of 2.5,5.0 and 10.0 μmol·L-1 were respectively given to BMSCs,and the cells treated with PBS only were used as control group.The cell viability and proliferation were detected with CCK-8 assay kit,the apoptosis of BMSCs was measured by Annexin V/PI apoptosis assay kit,while the PARP-1 gene expression was determined by reverse transcription real-time RT-PCR.Results Compared with PBS control group,the ability of cell proliferation raised obviously（P〈0.05）;the apoptotic rates of BMSCs in 5.0 and 10.0 μmol·L-1 HQ groups 48 h after exposure were significantly decreased（P〈0.05）.The expressions of PARP-1 in 2.5,5.0,and 10.0 μmol·L-1 HQ groups 48 h after exposure were 0.92,0.56（P〈0.05）,and 0.45 folds of control group（P〈0.05）.Conclusion Low level HQ could inhibit the apoptosis of BMSCs and PARP-1 gene expression,while promote the cell growth.