中文摘要：

目的：探讨槟榔碱对2型糖尿病大鼠肝脏胰岛素抵抗的影响及其机制。方法：采用高果糖饲料饲养Wistar大鼠12周制备2型糖尿病大鼠模型，实验动物随机分为5组（n=8）：对照组、模型组、模型＋不同浓度的槟榔碱（0，0．5，1，5mg／kg）组。4周后通过检测血糖、血脂、胰岛素、RT-PCR检测肝脏组成型雄甾烷受体（CAR）、孕甾烷x受体（PXR）、糖代谢相关基因：葡萄糖-6-磷酸酶（G6Pase）、磷酸烯醇式丙酮酸羧激酶（PEPCK）和炎症相关因子：白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）mRNA表达，Western blot检测大鼠肝内p-AKT和葡萄糖转运体4（GLUT4）蛋白表达。结果：1，5mg／kg槟榔碱显著降低糖尿病大鼠体重、空腹血糖、空腹胰岛素、血脂和糖代谢相关基因及炎症相关因子mRNA水平，提高CAR、PXR mRNA水平及p-AKT、GLUT4蛋白水平。结论：槟榔碱可能通过提高CAR和PXR的表达，导致肝脏糖代谢关键酶PEPCK、G6Pase基因表达或者炎性因子肿瘤坏死因子-α（TNF-α）、白介素-6（n-6）表达降低，改善2型糖尿病大鼠肝脏胰岛素抵抗。

英文摘要：

Objective： To explore the effects of arecohne on hepatic insulin resistance in type 2 diabetes rats and to elucidate its possible mechanism. Methods： Forty five Wistar rats were fed with high fructose diet for 12 weeks to induce type 2 diabetic rat model, rats were randomly divided into 5 groups（ n = 8） ： control group, model group and model group were treated with different dose （0,0.5, 1,5 mg/kg） of arecoline. After 4 weeks, the fasting blood glucose, blood lipid and insulin level measured , mRNA expression of liver constitutive androstane receptor （CAR）, pregnane X receptor （PXR）, glucose-6-phosphatase （G6Pase）, phosphoenolpyruvate carboxykinase （PEPCK）, interleukin- 6 （IL-6） and tumor necrosis factor-alpha （TNF-α） were detected by reverse transcription polymerase chain reaction （RT-PCR）, the protein expression of p-AKT and glucose transporter4 （GLUT4） were detected by Western blot. Results： 1,5 mg/kg arecoline could significantly decrease the level of fasting blood glucose , blood lipid , blood insulin level and liver G6Pase , PEPCK, ID6, TNF-α mRNA level in type 2 diabetes rats. 1,5 mg/kg arecoline also could significantly increase CAR, PXR mRNA level and p-AKT and GLUT4 protein expression. Conclusion： Arecoline improved hepatic insulin resistance in type 2 diabetes rats by increasing the mRNA levels of CAR and PXR leading to the decreased glucose metabolism and inflammation related genes expression.